iTRAQ analysis of a mouse acute myocardial infarction model reveals that vitamin D binding protein promotes cardiomyocyte apoptosis after hypoxia.
iTRAQ analysis of a mouse acute myocardial infarction model reveals that vitamin D binding protein promotes cardiomyocyte apoptosis after hypoxia.
复制标题
小鼠急性心肌梗死模型的 iTRAQ 分析揭示维生素 D 结合蛋白促进缺氧后心肌细胞凋亡
DOI：
10.18632/oncotarget.23025
复制
发表时间：
2018-01-05
期刊：
影响因子：
--
通讯作者：
Ma YT
中科院分区：
文献类型：
--
作者：
Wu Y;Liu F;Ma X;Adi D;Gai MT;Jin X;Yang YN;Huang Y;Xie X;Li XM;Fu ZY;Chen BD;Ma YT
急性心肌梗死（AMI）后蛋白质组谱的变化和重要蛋白质物种的作用仍然知之甚少。本研究通过结扎雄性C57B/6J小鼠左冠状动脉建立小鼠AMI模型，研究AMI后在蛋白水平上的分子变化。提取左心室总蛋白，采用相对定量和绝对定量（iTRAQ）等压标签技术进行定量分析。利用定量聚合酶链反应和western blot进一步验证重要基因的转录和蛋白水平。利用H9C2细胞构建氧糖剥夺/再灌注细胞模型，进一步验证缺氧后重要蛋白的表达模式和功能。AMI后鉴定出776个差异丰富蛋白，其中406个蛋白积累，370个蛋白减少。基因本体富集分析显示，最富集的分子功能类别项为结合，包括钙离子结合、GTP结合、肌动蛋白结合和脂质结合。缺血-缺氧后左室组织和H9C2细胞中维生素D结合蛋白（VDBP）及其相关蛋白的表达水平均升高。VDBP在H9C2细胞中的过表达降低了维生素D受体，促进了缺氧后细胞的凋亡率。我们的数据为AMI后蛋白质组谱的变化提供了新的见解，并表明VDBP可以促进缺氧后心肌细胞凋亡。
The proteome profile changes after acute myocardial infarction (AMI) and the roles played by important protein species remain poorly understood. Here, we constructed a mouse AMI model by ligating the left coronary artery of male C57B/6J mice to investigate the molecular changes after AMI on the protein level. Total proteins of the left ventricle were extracted and quantitatively analyzed by isobaric tags using relative and absolute quantitation (iTRAQ) technologies. The transcript and protein levels of important genes were further validated using quantitative polymerase chain reaction and western blot. An oxygen and glucose deprivation/reperfusion cell model was constructed using H9C2 cells to further validate the expression patterns and functions of important proteins after hypoxia. Seven hundred seventy-six proteins were identified as differentially abundant proteins after AMI, of which 406 were accumulated, and 370 were reduced. Gene ontology enrichment analysis showed that the most enriched molecular function category terms were binding, including calcium ion biding, GTP binding, actin binding and lipid binding. The expression levels of vitamin D binding protein (VDBP) and its related proteins were increased in both left ventricular tissue and H9C2 cells after ischemia-hypoxia. Overexpression of VDBP in H9C2 cells reduced vitamin D receptor and promoted the cell apoptosis rate after hypoxia. Our data provided new insights into proteome profile changes after AMI and indicated that VDBP could promote cardiomyocyte apoptosis after hypoxia.
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DOI：
10.1074/mcp.m114.040675
发表时间：
2014-10
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Peng Y;Gregorich ZR;Valeja SG;Zhang H;Cai W;Chen YC;Guner H;Chen AJ;Schwahn DJ;Hacker TA;Liu X;Ge Y
通讯作者：
Ge Y
影响因子：
2.5
作者：
Oda T;Matsumoto K
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Matsumoto K
影响因子：
1.1
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Asadi H;Abolfathi AA;Badalzadeh R;Majidinia M;Yaghoubi A;Asadi M;Yousefi B
通讯作者：
Yousefi B
DOI：
10.1097/00000433-200203000-00017
发表时间：
2002-03-01
影响因子：
1
作者：
Hutchins, KD;Skurnick, J;Natarajan, GA
通讯作者：
Natarajan, GA
agriGO：农业界的 GO 分析工具包
影响因子：
14.9
作者：
Du Z;Zhou X;Ling Y;Zhang Z;Su Z
通讯作者：
Su Z