The exosome regulates circadian gene expression in a posttranscriptional negative feedback loop.
The exosome regulates circadian gene expression in a posttranscriptional negative feedback loop.
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DOI：
10.1016/j.cell.2009.06.043
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发表时间：
2009-09-18
期刊：
影响因子：
64.5
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Guo J;Cheng P;Yuan H;Liu Y
The eukaryotic circadian oscillators consist of autoregulatory negative feedback loops. However, little is known about the role of post-transcriptional regulation of RNA in circadian oscillators. In the Neurospora circadian negative feedback loop, FRQ and FRH form the FFC complex that represses frq transcription. Here we show that FFC also binds frq RNA and interacts with the exosome to regulate frq RNA decay. Consequently, frq RNA is robustly rhythmic as it is more stable when FRQ levels are low. Silencing of RRP44, the catalytic subunit of the exosome, elevates frq RNA levels and impairs clock function. In addition, rrp44 is a clock-controlled gene and a direct target of the WHITE COLLAR complex, and RRP44 controls the circadian expression of at least one ccg. Taken together, these results suggest that FFC and the exosome are part of a post-transcriptional negative feedback loop that regulates frq transcript levels and the circadian output pathway.
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