Methodological comparison of different approaches to cultivate postnatal organotypic hippocampal cultures
Methodological comparison of different approaches to cultivate postnatal organotypic hippocampal cultures
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培养产后器官型海马培养物的不同方法的方法比较
DOI：
10.1101/2022.05.11.491471
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发表时间：
--
期刊：
影响因子：
--
通讯作者：
Klaus Funke
中科院分区：
文献类型：
--
作者：
Vivien Czapla;Pia Reiterer;Klaus Funke
为了在体外研究大脑的分子和细胞过程，必须建立出生后器官型海马切片的培养。由于培养结果不是最优的，因此必须对方法方法进行多次优化。最初，培养是通过滚筒管培养进行的，这是第一个开发的培养方法。随着培养物分离甚至溶解，使用插入物进行进一步培养，这是对滚轮管培养物的一种改进。在进一步的过程中，还涉及到使用不同的制备方法以及交换培养基和培养箱，以获得与期望相对应的器官培养结果。结果表明，在半透膜上培养比辊管培养有优势。一方面，由于机械影响，海马切片没有脱离和溶解，另一方面，与滚筒管培养相比，组织总体状况有所改善。此外，我们还发现，加速准备时间对神经元有积极的影响。此外，从记录的时间序列可以看出，培养时间越长，组织越稳定。如果海马体保持与内嗅皮层的连接，就不会发生沃勒氏变性，从而使细胞在出生后的培养中存活更长时间，从而加强组织。
To study molecular and cellular processes of the brainin vitro, the cultivation of postnatal organotypic hippocampal slices should be established. The methodological approach had to be optimized several times because the result of the cultivation was not optimal. Initially, cultivation was performed by roller tube culture, which is the first developed cultivation method for these cultures. As the cultures detached or even dissolved, further cultivation was carried out using inserts, which represent a modification of the roller tube cultures. In the further course it came also to the use of different preparation methods and to the exchange of the medium and the incubator, in order to obtain a result of the organ cultivation corresponding to the expectations. The results show that cultivation on semi-permeable membranes has an advantage over roller tube cultures. On the one hand, there is no detachment and dissolution of hippocampal slices due to the mechanical influence and on the other hand, the tissue shows an improvement in general condition compared to roller tube cultures. Furthermore, it was found that an acceleration of the preparation time has positive effects on the neurons. In addition, it could be shown by the recorded time series that a cultivation over a longer period of time stabilizes the tissue. If the hippocampi remain connected to the entorhinal cortex, wallerian degeneration does not occur, resulting in longer survival of the cells in the postnatal cultures and thus strengthening the tissue.
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影响因子：
0.8
作者：
CLAUSE, BT
通讯作者：
CLAUSE, BT
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DOI：
--
发表时间：
2006
期刊：
Theriogenology 65
影响因子：
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作者：
Ozawa M;Nagai T;Kaneko H;Noguchi J;Ohnuma K;Kikuchi K
通讯作者：
Kikuchi K
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影响因子：
3.7
作者：
Vlachos A;Becker D;Jedlicka P;Winkels R;Roeper J;Deller T
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Deller T
实验者：Zellkultur
DOI：
--
发表时间：
2020
期刊：
Experimentator
影响因子：
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作者：
Sabine Schmitz
通讯作者：
Sabine Schmitz
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影响因子：
4.8
作者：
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通讯作者：
X. Bo