cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons.
cAMP Signals in Drosophila Motor Neurons Are Confined to Single Synaptic Boutons.
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DOI：
10.1016/j.celrep.2016.09.090
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发表时间：
2016-10-25
期刊：
影响因子：
8.8
通讯作者：
Calebiro D
中科院分区：
文献类型：
--
作者：
Maiellaro I;Lohse MJ;Kittel RJ;Calebiro D
第二信使环AMP （cAMP）在突触可塑性中起重要作用。虽然有证据表明局部控制突触传递和可塑性，但尚不清楚是否存在类似的cAMP信号的空间限制。在这里，我们提出了cAMP对突触可塑性的位点特异性调节的可能的生物物理基础，cAMP是一种高度扩散的小分子，以局部和特异性的方式改变突触的生理。通过利用果蝇的章鱼胺能系统，该系统通过cAMP依赖途径介导结构突触可塑性，我们证明了单个运动神经元中存在微米尺寸的局部cAMP信号室。此外，我们提供的证据表明，章鱼胺受体的异质定位，加上磷酸二酯酶活性的局部差异，是轴突、细胞体和钮扣中观察到的cAMP信号传导差异的基础。扣、轴突和细胞体是独立的cAMP信号室受体和pde负责cAMP的区隔化cAMP不会从扣向细胞体传播，局部cAMP的增加为突触可塑性的位点特异性控制提供了基础Maiellaro等人发现，局部cAMP控制果蝇运动神经元的位点特异性突触可塑性。运动神经元中基因编码的荧光cAMP传感器的表达允许局部cAMP信号的可视化，并深入了解cAMP信号微域的形成。
The second messenger cyclic AMP (cAMP) plays an important role in synaptic plasticity. Although there is evidence for local control of synaptic transmission and plasticity, it is less clear whether a similar spatial confinement of cAMP signaling exists. Here, we suggest a possible biophysical basis for the site-specific regulation of synaptic plasticity by cAMP, a highly diffusible small molecule that transforms the physiology of synapses in a local and specific manner. By exploiting the octopaminergic system of Drosophila, which mediates structural synaptic plasticity via a cAMP-dependent pathway, we demonstrate the existence of local cAMP signaling compartments of micrometer dimensions within single motor neurons. In addition, we provide evidence that heterogeneous octopamine receptor localization, coupled with local differences in phosphodiesterase activity, underlies the observed differences in cAMP signaling in the axon, cell body, and boutons. Boutons, axon, and cell body are independent cAMP signaling compartments Receptors and PDEs are responsible for the compartmentalization of cAMP cAMP does not propagate from the bouton to the cell body Local cAMP increases provides a basis for site-specific control of synaptic plasticity Maiellaro et al. find that local cAMP controls site-specific synaptic plasticity in Drosophila motor neurons. The expression of a genetically encoded fluorescent cAMP sensor in motor neurons allows visualization of local cAMP signals and gives insight into the formation of cAMP signaling microdomains.
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影响因子：
8.8
作者：
Ljaschenko, Dmitrij;Ehmann, Nadine;Kittel, Robert J.
通讯作者：
Kittel, Robert J.
DOI：
10.1073/pnas.1100247108
发表时间：
2011-08-16
影响因子：
11.1
作者：
Nicol, Xavier;Hong, Kwan Pyo;Spitzer, Nicholas C.
通讯作者：
Spitzer, Nicholas C.
影响因子：
25
作者：
Peled ES;Isacoff EY
通讯作者：
Isacoff EY
影响因子：
16.6
作者：
Ehmann, Nadine;van de Linde, Sebastian;Alon, Amit;Ljaschenko, Dmitrij;Keung, Xi Zhen;Holm, Thorge;Rings, Annika;DiAntonio, Aaron;Hallermann, Stefan;Ashery, Uri;Heckmann, Manfred;Sauer, Markus;Kittel, Robert J.
通讯作者：
Kittel, Robert J.
影响因子：
16.6
作者：
Li, Lu;Gervasi, Nicolas;Girault, Jean-Antoine
通讯作者：
Girault, Jean-Antoine