Polyamines and linear DNA mediate bacterial threat assessment of bacteriophage infection.
Polyamines and linear DNA mediate bacterial threat assessment of bacteriophage infection.
复制标题
DOI：
10.1073/pnas.2216430120
复制
发表时间：
2023-02-28
影响因子：
11.1
通讯作者：
Secor, Patrick R.
中科院分区：
文献类型：
--
作者：
de Mattos, Camilla D.;Faith, Dominick R.;Nemudryi, Artem A.;Schmidt, Amelia K.;Bublitz, DeAnna C.;Hammond, Lauren;Kinnersley, Margie A.;Schwartzkopf, Caleb M.;Robinson, Autumn J.;Joyce, Alex;Michaels, Lia A.;Brzozowski, Robert S.;Coluccio, Alison;Xing, Denghui David;Uchiyama, Jumpei;Jennings, Laura K.;Eswara, Prahathees;Wiedenheft, Blake;Secor, Patrick R.
当细胞溶解时，危险信号被释放到环境中，警告邻近的细胞附近的危险。然而，作为细菌危险信号的分子定义不清，危险信号如何影响细菌对噬菌体感染等威胁的反应也是未知的。在这里，我们证明了溶解细菌释放的多胺被邻近细胞内化。在没有噬菌体感染的情况下，细胞内多胺水平迅速恢复到基线。当噬菌体感染细胞时，它们注入线性DNA，导致细胞内多胺水平保持高水平，干扰噬菌体DNA复制。我们的研究结果表明，多胺释放的裂解细胞和线性DNA与噬菌体感染的危险信号，指导细菌威胁评估噬菌体感染。监测细胞外环境中的危险信号是细胞存活的一个关键方面。然而，死亡细菌释放的危险信号和细菌用于威胁评估的机制在很大程度上仍未被探索。在这里，我们表明，铜绿假单胞菌细胞裂解释放多胺，随后通过依赖于Gac/Rsm信号传导的机制被存活的细胞吸收。虽然细胞内多胺在存活细胞中尖峰，但这种尖峰的持续时间根据细胞的感染状态而变化。在噬菌体感染的细胞中，细胞内多胺维持在高水平，这抑制了噬菌体基因组的复制。许多噬菌体包装线性DNA基因组，线性DNA足以触发细胞内多胺积累，这表明线性DNA被认为是第二个危险信号。总的来说，这些结果证明了死亡细胞释放的多胺如何与线性DNA一起允许铜绿假单胞菌对细胞损伤进行威胁评估。
When cells lyse, danger signals are released into the environment that warn adjacent cells of nearby danger. However, the molecules that serve as bacterial danger signals are poorly defined, and how danger signals affect bacterial responses to threats such as bacteriophage infection are unknown. Here, we demonstrate that polyamines released by lysed bacteria are internalized by adjacent cells. In the absence of bacteriophage infection, intracellular polyamine levels quickly return to baseline. When bacteriophage infect a cell, they inject linear DNA that causes intracellular polyamine levels to remain high, interfering with bacteriophage DNA replication. Our results indicate that polyamines released by lysed cells and linear DNA associated with bacteriophage infection are danger signals that guide bacterial threat assessment of bacteriophage infection. Monitoring the extracellular environment for danger signals is a critical aspect of cellular survival. However, the danger signals released by dying bacteria and the mechanisms bacteria use for threat assessment remain largely unexplored. Here, we show that lysis of Pseudomonas aeruginosa cells releases polyamines that are subsequently taken up by surviving cells via a mechanism that relies on Gac/Rsm signaling. While intracellular polyamines spike in surviving cells, the duration of this spike varies according to the infection status of the cell. In bacteriophage-infected cells, intracellular polyamines are maintained at high levels, which inhibits replication of the bacteriophage genome. Many bacteriophages package linear DNA genomes and linear DNA is sufficient to trigger intracellular polyamine accumulation, suggesting that linear DNA is sensed as a second danger signal. Collectively, these results demonstrate how polyamines released by dying cells together with linear DNA allow P. aeruginosa to make threat assessments of cellular injury.
登录
查看更多内容
影响因子：
28.3
作者：
Herrero del Valle, Alba;Seip, Britta;Innis, C. Axel
通讯作者：
Innis, C. Axel
影响因子：
14.8
作者：
Hmelo LR;Borlee BR;Almblad H;Love ME;Randall TE;Tseng BS;Lin C;Irie Y;Storek KM;Yang JJ;Siehnel RJ;Howell PL;Singh PK;Tolker-Nielsen T;Parsek MR;Schweizer HP;Harrison JJ
通讯作者：
Harrison JJ
影响因子：
16.6
作者：
Francis VI;Waters EM;Finton-James SE;Gori A;Kadioglu A;Brown AR;Porter SL
通讯作者：
Porter SL
影响因子：
4.4
作者：
Fazli, Mustafa;Harrison, Joe J.;Tolker-Nielsen, Tim
通讯作者：
Tolker-Nielsen, Tim
影响因子：
3.7
作者：
HERSHEY, AD
通讯作者：
HERSHEY, AD