Multiple communication mechanisms between sensor kinases are crucial for virulence in Pseudomonas aeruginosa.
Multiple communication mechanisms between sensor kinases are crucial for virulence in Pseudomonas aeruginosa.
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DOI：
10.1038/s41467-018-04640-8
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发表时间：
2018-06-07
影响因子：
16.6
通讯作者：
Porter SL
中科院分区：
文献类型：
--
作者：
Francis VI;Waters EM;Finton-James SE;Gori A;Kadioglu A;Brown AR;Porter SL
细菌和许多非后生动物真核生物使用包括传感器激酶（SK）和反应调节剂（RR）的双组分系统（TCS）来响应压力和威胁。多激酶网络，其中多个SK一起工作，检测和整合不同的信号，以控制重要的生活方式决定，如孢子形成和毒力。在这里，我们研究了铜绿假单胞菌GacS和RetS两种SK之间的相互作用，它们控制着急性和慢性毒力之间的转换。我们证明了RetS减弱GacS信号传导的三种机制：RetS从GacS-P中摄取磷酰基; RetS对GacS-P的受体结构域具有发射磷酸酶活性; RetS抑制GacS自磷酸化。这些机制在体内和感染过程中发挥着重要作用，并使SK的信号处理达到前所未有的程度，这些信号处理可能在其他多激酶网络中被利用。细菌使用由传感器激酶（SK）和响应调节剂组成的双组分系统来响应压力。在这里，弗朗西斯等人揭示了一对SK之间的三种特定相互作用机制，这些机制对于调节病原体铜绿假单胞菌的毒力非常重要。
Bacteria and many non-metazoan Eukaryotes respond to stresses and threats using two-component systems (TCSs) comprising sensor kinases (SKs) and response regulators (RRs). Multikinase networks, where multiple SKs work together, detect and integrate different signals to control important lifestyle decisions such as sporulation and virulence. Here, we study interactions between two SKs from Pseudomonas aeruginosa, GacS and RetS, which control the switch between acute and chronic virulence. We demonstrate three mechanisms by which RetS attenuates GacS signalling: RetS takes phosphoryl groups from GacS-P; RetS has transmitter phosphatase activity against the receiver domain of GacS-P; and RetS inhibits GacS autophosphorylation. These mechanisms play important roles in vivo and during infection, and exemplify an unprecedented degree of signal processing by SKs that may be exploited in other multikinase networks. Bacteria respond to stresses using two-component systems consisting of sensor kinases (SKs) and response regulators. Here, Francis et al. reveal three specific interaction mechanisms between a pair of SKs that are important for regulation of virulence in the pathogen Pseudomonas aeruginosa.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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作者：
Francis VI;Stevenson EC;Porter SL
通讯作者：
Porter SL