Interruptions in gene expression drive highly expressed operons to the leading strand of DNA replication.
Interruptions in gene expression drive highly expressed operons to the leading strand of DNA replication.
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DOI：
10.1093/nar/gki638
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发表时间：
2005
影响因子：
14.9
通讯作者：
Arkin, AP
中科院分区：
文献类型：
--
作者：
Price, MN;Alm, EJ;Arkin, AP
在细菌中，大多数基因位于复制的主导链上，这种现象归因于 DNA 和 RNA 聚合酶之间的碰撞。在大肠杆菌中，只有当活跃转录基因编码在滞后链上时，这些碰撞才会减缓复制叉通过活跃转录基因的运动。然而，对于两条链上的基因来说，这些碰撞会切断新生转录本并中断基因表达。基于这些观察，我们提出了一个新的理论来解释链偏差：其表达对适应性很重要的基因被选择为主导链，因为这减少了这些中断的持续时间。我们的理论预测，受到较长时间中断的多基因操纵子应该比单例转录本更强烈地选择主导链。我们证明，即使在控制了强烈偏向于前导链的必需基因在操纵子中出现的趋势之后，情况也是如此。我们的理论还预测，与链偏倚相关的其他因素应该对操纵子中的基因产生更强的影响。我们发现表达水平和系统发育普遍性与必需和非必需基因的链偏倚相关，但仅限于操纵子中的基因。
In bacteria, most genes are on the leading strand of replication, a phenomenon attributed to collisions between the DNA and RNA polymerases. In Escherichia coli, these collisions slow the movement of the replication fork through actively transcribed genes only if they are coded on the lagging strand. For genes on both strands, however, these collisions sever nascent transcripts and interrupt gene expression. Based on these observations, we propose a new theory to explain strand bias: genes whose expression is important for fitness are selected to the leading strand because this reduces the duration of these interruptions. Our theory predicts that multi-gene operons, which are subject to longer interruptions, should be more strongly selected to the leading strand than singleton transcripts. We show that this is true even after controlling for the tendency for essential genes, which are strongly biased to the leading strand, to occur in operons. Our theory also predicts that other factors that are associated with strand bias should have stronger effects for genes that are in operons. We find that expression level and phylogenetic ubiquity are correlated with strand bias for both essential and non-essential genes, but only for genes in operons.
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影响因子：
14.9
作者：
Price MN;Huang KH;Alm EJ;Arkin AP
通讯作者：
Arkin AP
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
14.9
作者：
Ermolaeva, MD;White, O;Salzberg, SL
通讯作者：
Salzberg, SL
影响因子：
11.4
作者：
Pál, C;Hurst, LD
通讯作者：
Hurst, LD
影响因子：
14.9
作者：
Gollub, J;Ball, CA;Sherlock, G
通讯作者：
Sherlock, G