SmTRC1, a novel Schistosoma mansoni DNA transposon, discloses new families of animal and fungi transposons belonging to the CACTA superfamily.
SmTRC1, a novel Schistosoma mansoni DNA transposon, discloses new families of animal and fungi transposons belonging to the CACTA superfamily.
复制标题
DOI：
10.1186/1471-2148-6-89
复制
发表时间：
2006-11-07
影响因子：
3.4
通讯作者：
Verjovski-Almeida S
中科院分区：
文献类型：
--
作者：
DeMarco R;Venancio TM;Verjovski-Almeida S
CACTA（也称为En/Spm）超家族的DNA转座子在其末端反向重复序列（TIR）中含有核心序列CACTA，并且迄今为止仅在植物中描述。大转录组和基因组序列数据最近已成为公开的曼氏血吸虫，二代血吸虫，是一个主要的病原体在人类血吸虫病，并提供了一个全面的知识库，发现新的基因和重复元件。尽管S. mansoni，到目前为止，在这种生物体中只报道了一个属于Merlin家族的仅DNA转座子。我们描述了一个小说S。mansoni转座子命名为SmTRC 1。mansoni转座子与CACTA 1相关，是一种与植物CACTA转座子共享几个特征的元件。Southern杂交表明，在S. mansoni基因组。使用基因组PCR，然后克隆和测序，我们扩增并表征了该元件的全长和截短拷贝。使用S. mansoni mRNA的克隆和测序揭示了该转座子的几个选择性剪接转录本，导致编码不同蛋白质的不同ORF。有趣的是，对动物和真菌的完整基因组的调查揭示了其他几个新的TRC元件，表明属于CACTA超家族的DNA转座子的新家族，这些家族以前在这些王国中没有报道过。S. mansoni TIR是CCC，并且它们与昆虫埃及伊蚊和赤拟谷盗的TIR中的TIR相同，表明动物TRC可能展示CCC核心序列。从S. mansoni具有多种特征，如产生多个选择性剪接的转录本，在末端存在反向重复序列，并存在转座酶_21结构域，这些特征表明与木兰门的CACTA转座子有较远的关系。来自其他后生动物和真菌的几个序列编码的蛋白质与SmTRC 1编码的蛋白质相似，这表明这些元件具有共同的祖先，并表明在真后生动物，真菌和植物分离之前通过垂直传播进行遗传。
The CACTA (also called En/Spm) superfamily of DNA-only transposons contain the core sequence CACTA in their Terminal Inverted Repeats (TIRs) and so far have only been described in plants. Large transcriptome and genome sequence data have recently become publicly available for Schistosoma mansoni, a digenetic blood fluke that is a major causative agent of schistosomiasis in humans, and have provided a comprehensive repository for the discovery of novel genes and repetitive elements. Despite the extensive description of retroelements in S. mansoni, just a single DNA-only transposon belonging to the Merlin family has so far been reported in this organism. We describe a novel S. mansoni transposon named SmTRC1, for S. mansoni Transposon Related to CACTA 1, an element that shares several characteristics with plant CACTA transposons. Southern blotting indicates approximately 30–300 copies of SmTRC1 in the S. mansoni genome. Using genomic PCR followed by cloning and sequencing, we amplified and characterized a full-length and a truncated copy of this element. RT-PCR using S. mansoni mRNA followed by cloning and sequencing revealed several alternatively spliced transcripts of this transposon, resulting in distinct ORFs coding for different proteins. Interestingly, a survey of complete genomes from animals and fungi revealed several other novel TRC elements, indicating new families of DNA transposons belonging to the CACTA superfamily that have not previously been reported in these kingdoms. The first three bases in the S. mansoni TIR are CCC and they are identical to those in the TIRs of the insects Aedes aegypti and Tribolium castaneum, suggesting that animal TRCs may display a CCC core sequence. The DNA-only transposable element SmTRC1 from S. mansoni exhibits various characteristics, such as generation of multiple alternatively-spliced transcripts, the presence of terminal inverted repeats at the extremities of the elements flanked by direct repeats and the presence of a Transposase_21 domain, that suggest a distant relationship to CACTA transposons from Magnoliophyta. Several sequences from other Metazoa and Fungi code for proteins similar to those encoded by SmTRC1, suggesting that such elements have a common ancestry, and indicating inheritance through vertical transmission before separation of the Eumetazoa, Fungi and Plants.
登录
查看更多内容
影响因子：
1.5
作者：
Kapitonov, VV;Jurka, J
通讯作者：
Jurka, J
影响因子：
11.4
作者：
FREY, M;REINECKE, J;GIERL, A
通讯作者：
GIERL, A
影响因子：
5.4
作者：
DeMarco, R;Kowaltowski, AT;Verjovski-Almeida, S
通讯作者：
Verjovski-Almeida, S
DOI：
10.1073/pnas.94.15.7704
发表时间：
1997-07-22
影响因子：
11.1
作者：
Kidwell, MG;Lisch, D
通讯作者：
Lisch, D
DOI：
10.1016/j.bbrc.2005.05.080
发表时间：
2005-07-22
影响因子：
3.1
作者：
DeMarco, R;Machado, AA;Verjovski-Almeida, S
通讯作者：
Verjovski-Almeida, S