AoSsk1, a Response Regulator Required for Mycelial Growth and Development, Stress Responses, Trap Formation, and the Secondary Metabolism in Arthrobotrys oligospora.
AoSsk1, a Response Regulator Required for Mycelial Growth and Development, Stress Responses, Trap Formation, and the Secondary Metabolism in Arthrobotrys oligospora.
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DOI：
10.3390/jof8030260
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发表时间：
2022-03-03
期刊：
影响因子：
--
通讯作者：
Yang JK
中科院分区：
文献类型：
--
作者：
Jiang KX;Liu QQ;Bai N;Zhu MC;Zhang KQ;Yang JK
Ssk1 是双组分信号系统的反应调节因子，在真菌高渗应激的细胞反应中发挥着重要作用。在此，通过基因破坏和多表型比较，对线虫捕获真菌寡孢节孢菌中 ssk1 (Aossk1) 的直向同源物进行了表征。 Aossk1 的缺失导致生长缺陷、菌丝细胞变形和肿胀、菌丝隔膜增大和细胞核萎缩。与野生型（WT）菌株相比，ΔAossk1突变体菌丝细胞中自噬体和脂滴的数量减少，而体积却显着增加。 Aossk1 破坏导致分生孢子产量减少 95%，并且对渗透和氧化应激的耐受性显着缺陷。同时，与WT菌株相比，ΔAossk1突变体中几个孢子形成相关基因的转录水平显着降低，包括abaA、brlA、flbC、fluG和rodA。此外，Aossk1 的缺失导致陷阱形成和捕食效率显着增加。此外，与 WT 菌株相比，ΔAossk1 突变体中的许多代谢物显着下调。我们的结果强调，AoSsk1 是无性发育、应激反应、次级代谢和致病性的关键调节因子，可用于探索线虫捕获真菌的陷阱形成和生活方式转换的调节机制。
Ssk1, a response regulator of the two-component signaling system, plays an important role in the cellular response to hyperosmotic stress in fungi. Herein, an ortholog of ssk1 (Aossk1) was characterized in the nematode-trapping fungus Arthrobotrys oligospora using gene disruption and multi-phenotypic comparison. The deletion of Aossk1 resulted in defective growth, deformed and swollen hyphal cells, an increased hyphal septum, and a shrunken nucleus. Compared to the wild-type (WT) strain, the number of autophagosomes and lipid droplets in the hyphal cells of the ΔAossk1 mutant decreased, whereas their volumes considerably increased. Aossk1 disruption caused a 95% reduction in conidial yield and remarkable defects in tolerance to osmotic and oxidative stress. Meanwhile, the transcript levels of several sporulation-related genes were significantly decreased in the ΔAossk1 mutant compared to the WT strain, including abaA, brlA, flbC, fluG, and rodA. Moreover, the loss of Aossk1 resulted in a remarkable increase in trap formation and predation efficiency. In addition, many metabolites were markedly downregulated in the ΔAossk1 mutant compared to the WT strain. Our results highlight that AoSsk1 is a crucial regulator of asexual development, stress responses, the secondary metabolism, and pathogenicity, and can be useful in probing the regulatory mechanism underlying the trap formation and lifestyle switching of nematode-trapping fungi.
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