Mapping resistance to powdery mildew in barley reveals a large-effect nonhost resistance QTL.
Mapping resistance to powdery mildew in barley reveals a large-effect nonhost resistance QTL.
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DOI：
10.1007/s00122-018-3055-0
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Niks RE
中科院分区：
文献类型：
--
作者：
Romero CCT;Vermeulen JP;Vels A;Himmelbach A;Mascher M;Niks RE
在大麦中定位了对非适应性白粉病的抗性因子。一些QTL似乎只对非适应的霉菌有效，而另一些QTL也对适应的形式起到防御作用。非寄主抗性的持久性和有效性表明，依赖于对这种类型抗性关键方面的阐明，在作物育种中具有很好的实际应用前景。研究了哪些遗传因素决定了大麦的非寄主地位。白粉病(Blumeria Graminis)。我们开始验证与非寄主抗性相关的基因是否具有广泛的有效谱，以及非寄主抗性基因是否赋予大麦适应白粉病的抗性。两个大麦品系SusBgtSC和SusBgtDC对小麦白粉病B.graminis f.sp.将小麦(Tritici(BGT))与瓦达小麦(Cv Vada)杂交，形成两个作图群体。评估了每个种群对四个白粉菌的感染水平，并进行了QTL定位分析。我们的结果证明了非寄主抗性的多基因遗传，一些QTL只对非适应霉菌有效，而另一些QTL对适应和非适应形式起作用。对非寄主相互作用的组织学分析表明，大多数穿透尝试都与乳突有关，也表明吸器建立和分生孢子形成时有独立的防御层。大麦对白粉病的非寄主抗性主要依赖于非过敏性机制。定位在1.4 cM区间的大效非寄主抗性QTL适合作图克隆。本文的在线版本(10.1007/s00122-0183055-0)包含向授权用户提供的补充材料。
Resistance factors against non-adapted powdery mildews were mapped in barley. Some QTLs seem effective only to non-adapted mildews, while others also play a role in defense against the adapted form. The durability and effectiveness of nonhost resistance suggests promising practical applications for crop breeding, relying upon elucidation of key aspects of this type of resistance. We investigated which genetic factors determine the nonhost status of barley (Hordeum vulgare L.) to powdery mildews (Blumeria graminis). We set out to verify whether genes involved in nonhost resistance have a wide effectiveness spectrum, and whether nonhost resistance genes confer resistance to the barley adapted powdery mildew. Two barley lines, SusBgtSC and SusBgtDC, with some susceptibility to the wheat powdery mildew B. graminis f.sp. tritici (Bgt) were crossed with cv Vada to generate two mapping populations. Each population was assessed for level of infection against four B. graminis ff.spp, and QTL mapping analyses were performed. Our results demonstrate polygenic inheritance for nonhost resistance, with some QTLs effective only to non-adapted mildews, while others play a role against adapted and non-adapted forms. Histology analyses of nonhost interaction show that most penetration attempts are stopped in association with papillae, and also suggest independent layers of defence at haustorium establishment and conidiophore formation. Nonhost resistance of barley to powdery mildew relies mostly on non-hypersensitive mechanisms. A large-effect nonhost resistance QTL mapped to a 1.4 cM interval is suitable for map-based cloning. The online version of this article (10.1007/s00122-018-3055-0) contains supplementary material, which is available to authorized users.
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影响因子：
2.3
作者：
Kolmer, James A.;Singh, Ravi P.;Lagudah, Evans S.
通讯作者：
Lagudah, Evans S.
影响因子：
18
作者：
Du, Juan;Verzaux, Estelle;Vleeshouwers, Vivianne G. A. A.
通讯作者：
Vleeshouwers, Vivianne G. A. A.
影响因子：
56.9
作者：
Krattinger, Simon G.;Lagudah, Evans S.;Keller, Beat
通讯作者：
Keller, Beat
影响因子：
5.4
作者：
GIESE, H;HOLMJENSEN, AG;JENSEN, J
通讯作者：
JENSEN, J
影响因子：
3.7
作者：
Elshire RJ;Glaubitz JC;Sun Q;Poland JA;Kawamoto K;Buckler ES;Mitchell SE
通讯作者：
Mitchell SE