Enhancement and Repair of Degenerative Intervertebral Disc in Rats Using Platelet-Rich Plasma/Ferulic Acid Hydrogel.
Enhancement and Repair of Degenerative Intervertebral Disc in Rats Using Platelet-Rich Plasma/Ferulic Acid Hydrogel.
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DOI：
10.1177/19476035231157341
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发表时间：
2023-12
期刊：
影响因子：
2.8
通讯作者：
Zhu, Yong
中科院分区：
文献类型：
--
作者：
Chai, Qiang;Zhang, Baining;Da, Yifeng;Wang, Wenlei;Gao, Yidan;Yao, Mingyu;Zhu, He;Yang, Xuejun;Zhu, Yong
关键词：
椎间盘退变（IDD）疾病是导致腰痛的最常见临床病症之一。本研究的主要目的是结合生物工程技术，研究富血小板血浆（PRP）和阿魏酸（FA）水凝胶复合物对大鼠退变椎间盘的修复作用，这可能为未来IDD的治疗提供有力的理论依据。
将45只雄性Sprague - Dawley大鼠随机分为A - F组；在注射后0、4和8周对每组进行磁共振成像（MRI）检查；处死后获取椎间盘组织。通过苏木精 - 伊红染色、Masson染色、脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（TUNEL）染色和蛋白质印迹法（Western blot）观察每组椎间盘的组织形态学、细胞凋亡和蛋白质合成情况。
所有组的释放浓度在12小时达到峰值，同时水凝胶/PRP/FA组浓度最高。MTT实验表明水凝胶/PRP/FA具有良好的细胞相容性。动物实验结果显示水凝胶/PRP/FA对大鼠退变椎间盘有良好的效果。
富含PRP/FA的水凝胶复合物在促进大鼠细胞外基质合成、强化和修复退变椎间盘方面发挥积极作用。
Intervertebral degenerative disc (IDD) disease is one of the most common clinical conditions causing low back pain. The main objective of this study was to investigate the repair effect of platelet-rich plasma (PRP) and ferulic acid (FA) hydrogel compound on degenerative discs in rats in combination with bioengineering technology, which may provide a strong theoretical basis for the future treatment of IDD. Forty-five male Sprague-Dawley rats were randomly divided into groups A-F; MRI was performed in each group at 0, 4, and 8 weeks after injection; and disc tissues were obtained after executing the animals. The histomorphology, apoptosis, and protein synthesis of intervertebral discs in each group were observed by hematoxylin-eosin, Masson, terminal deoxynucleotidyl transferase dUTP nick end labeling staining, and Western blot. The release concentration of all groups reached the peak at 12 hours, and the highest concentration was found in the hydrogel/PRP/FA group at the same time. The MTT assay showed that hydrogel/PRP/FA is well-cytocompatible. The results of animal experiments show that hydrogel/PRP/FA has a good effect on degenerative intervertebral disc in rats. PRP/FA-rich hydrogel compound plays an active role in promoting extracellular matrix synthesis, strengthening and repairing degenerated intervertebral discs in rats.
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影响因子：
14
作者：
Bowles RD;Setton LA
通讯作者：
Setton LA
影响因子：
4
作者：
Ganesan, Ramamoorthi;Rasool, Mahaboobkhan
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5.6
作者：
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影响因子：
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作者：
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通讯作者：
Lutz, Gregory E.
DOI：
10.1177/0954411916681597
发表时间：
2017-02-01
影响因子：
1.8
作者：
Nikkhoo, Mohammad;Wang, Jaw-Lin;Khalaf, Kinda
通讯作者：
Khalaf, Kinda