Constituents of Propolis: Chrysin, Caffeic Acid, p-Coumaric Acid, and Ferulic Acid Induce PRODH/POX-Dependent Apoptosis in Human Tongue Squamous Cell Carcinoma Cell (CAL-27).
Constituents of Propolis: Chrysin, Caffeic Acid, p-Coumaric Acid, and Ferulic Acid Induce PRODH/POX-Dependent Apoptosis in Human Tongue Squamous Cell Carcinoma Cell (CAL-27).
复制标题
DOI：
10.3389/fphar.2018.00336
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Miltyk W
中科院分区：
文献类型：
--
作者：
Celińska-Janowicz K;Zaręba I;Lazarek U;Teul J;Tomczyk M;Pałka J;Miltyk W
蜂胶具有多种治疗特性，包括抗癌活性。这些活性归因于多酚的作用。先前已经证明，蜂胶乙醇提取物中最丰富的多酚化合物之一是白杨素、咖啡酸、对香豆酸和阿魏酸。尽管它们对人舌鳞状细胞癌细胞（CAL - 27）的促凋亡活性先前已被确定，但这一过程的详细机制仍不清楚。考虑到脯氨酸代谢以及脯氨酸脱氢酶/脯氨酸氧化酶（PRODH/POX）在癌细胞存活/凋亡调节中的关键作用，我们研究了多酚处理的CAL - 27细胞中的这些过程。所有研究的多酚都引发了抗增殖活性，伴随着CAL - 27细胞中PRODH/POX、P53、活性胱天蛋白酶 - 3和 - 9表达增加，以及胶原蛋白生物合成、氨酰脯氨酸酶活性和脯氨酸浓度降低。这些数据表明，蜂胶中的多酚通过上调线粒体脯氨酸降解以及下调用于胶原蛋白生物合成的脯氨酸利用，诱导PRODH/POX依赖性凋亡。
Propolis evokes several therapeutic properties, including anticancer activity. These activities are attributed to the action of polyphenols. Previously it has been demonstrated, that one of the most abundant polyphenolic compounds in ethanolic extracts of propolis are chrysin, caffeic acid, p-coumaric acid, and ferulic acid. Although their pro-apoptotic activity on human tongue squamous cell carcinoma cells (CAL-27) was established previously, the detailed mechanism of this process remains unclear. Considering the crucial role of proline metabolism and proline dehydrogenase/proline oxidase (PRODH/POX) in the regulation of cancer cell survival/apoptosis, we studied these processes in polyphenol-treated CAL-27 cells. All studied polyphenols evoked anti-proliferative activity, accompanied by increased PRODH/POX, P53, active caspases-3 and -9 expressions and decreased collagen biosynthesis, prolidase activity and proline concentration in CAL-27 cells. These data suggest that polyphenols of propolis induce PRODH/POX-dependent apoptosis through up-regulation of mitochondrial proline degradation and down-regulation of proline utilization for collagen biosynthesis.
登录
查看更多内容
影响因子：
--
作者：
Bankova V;Popova M;Trusheva B
通讯作者：
Trusheva B
影响因子：
--
作者：
Kubiliene L;Laugaliene V;Pavilonis A;Maruska A;Majiene D;Barcauskaite K;Kubilius R;Kasparaviciene G;Savickas A
通讯作者：
Savickas A
影响因子：
1.3
作者：
Agarwal, Pankaj;Ballabh, Ram
通讯作者：
Ballabh, Ram
影响因子：
3.5
作者：
Phang, James M.;Donald, Steven P.;Liu, Yongmin
通讯作者：
Liu, Yongmin
影响因子：
8
作者：
Liu, Y.;Borchert, G. L.;Phang, J. M.
通讯作者：
Phang, J. M.