Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolates.
Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolates.
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DOI：
10.1186/1297-9716-44-87
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发表时间：
2013-10-01
影响因子：
4.4
通讯作者：
Dixon LK
中科院分区：
文献类型：
--
作者：
Fishbourne E;Hutet E;Abrams C;Cariolet R;Le Potier MF;Takamatsu HH;Dixon LK
比较了非洲猪瘟病毒高毒力分离株和低毒力分离株感染猪后全血中趋化因子及其受体表达的变化。在两个实验中，CCL2、CCL3L1、CCL4、CXCL10、CCR1和CCR5的mRNAs水平在感染后至少一个时间点显著升高，其中一个实验中CCL5、CCR9和CXCR4的mRNA量显著增加。结果表明，感染猪后，CXCL10和CCL2的mRNAs增加幅度最大。感染猪的CXCL10基因表达水平比未感染猪高达15倍。在高毒力贝宁97/1分离株感染的猪血清中也检测到CXCL10蛋白。与弱毒株OURT88/3相比，高毒力贝宁97/1株感染猪的CCL2mRNA水平升高，这与感染该毒株的猪血清中CCL2蛋白水平增加30倍以上有关。贝宁97/1感染猪去纤化血浆中总的趋化活性化合物在感染后3天(Dpi)增加，而用正常猪白细胞体外趋化试验测得总趋化活性化合物减少7 dpi。CXCL10水平的升高可能有助于激活淋巴细胞对Th1表型的启动或诱导T淋巴细胞的凋亡。巨噬细胞趋化剂CCL2水平的增加可能会导致单核细胞从骨髓中招募的增加，从而增加易受感染的细胞池。
Modulation of the expression of chemokines and chemokine receptors in whole blood was compared following infection of pigs with high and low virulence isolates of African swine fever virus. Levels of mRNAs for CCL2, CCL3L1, CCL4, CXCL10, CCR1 and CCR5 were significantly increased in at least one time point following infection in two experiments and CCL5, CCR9 and CXCR4 mRNA were significantly increased in one of the experiments. The results showed that greatest fold increases in mRNAs for CXCL10 and CCL2 were observed following infection of pigs. CXCL10 mRNA was increased by up to 15 fold in infected compared to uninfected pigs. CXCL10 protein was also detected in serum from pigs infected with the high virulence Benin 97/1 isolate. Levels of CCL2 mRNA were increased in pigs infected with high virulence Benin 97/1 isolate compared to low virulence OURT88/3 isolate and this correlated with an increase of greater than 30 fold in levels of CCL2 protein detected in serum from pigs infected with this isolate. An increase in overall chemotaxis active compounds in defibrinated plasma samples from Benin 97/1 infected pigs was observed at 3 days post-infection (dpi) and a decrease by 7 dpi as measured by chemotaxis assay using normal pig leucocytes in vitro. Increased levels of CXCL10 may either contribute to the activation of lymphocyte priming toward the Th1 phenotype or induction of T lymphocyte apoptosis. Increased levels of CCL2, a chemoattractant for macrophages, may result in increased recruitment of monocytes from bone marrow thus increasing the pool of cells susceptible to infection.
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影响因子：
3.7
作者：
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Horvat B
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Revilla, Yolanda
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作者：
Oura, CAL;Denyer, MS;Parkhouse, RME
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Parkhouse, RME