Integration of drug, protein, and gene delivery systems with regenerative medicine.
Integration of drug, protein, and gene delivery systems with regenerative medicine.
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DOI：
10.1007/s13346-013-0165-8
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发表时间：
2015-04
影响因子：
5.4
通讯作者：
Leong, Kam W.
中科院分区：
文献类型：
--
作者：
Lorden, Elizabeth R.;Levinson, Howard M.;Leong, Kam W.
再生医学有潜力彻底改变卫生保健领域，从被动反应到预防和恢复。干细胞生物学和细胞重编程方面令人兴奋的进展推动了这一领域的发展。以小分子药物、生长因子、锌指蛋白转录因子和核酸酶、转录激活因子样效应核酸酶、单克隆抗体、质粒DNA、适体或RNA干扰剂等形式出现的生化线索，在影响干细胞分化和组织再生结果方面发挥着重要作用。这些生化因子中有许多是脆弱的，必须在细胞内的分子水平上起作用。它们需要一种有效的递送系统，可以采用支架（例如水凝胶和静电纺纤维）、载体（病毒和非病毒）、纳米和微粒或转基因细胞的形式。本文将对再生医学背景下药物、蛋白质和基因传递的历史和最新技术进行综述。接下来，我们将介绍如何应用输送技术来促进未愈合伤口的血管生成或防止年龄相关性黄斑变性的血管生成。最后，我们将简要讨论这些新疗法的临床转化从实验室到床边的调控途径。
Regenerative medicine has the potential to drastically change the field of health care from reactive to preventative and restorative. Exciting advances in stem cell biology and cellular reprogramming have fueled the progress of this field. Biochemical cues in the form of small molecule drugs, growth factors, zinc finger protein transcription factors and nucleases, transcription activator-like effector nucleases, monoclonal antibodies, plasmid DNA, aptamers, or RNA interference agents can play an important role to influence stem cell differentiation and the outcome of tissue regeneration. Many of these biochemical factors are fragile and must act intracellularly at the molecular level. They require an effective delivery system, which can take the form of a scaffold (e.g. hydrogels and electrospun fibers), carrier (viral and nonviral), nano- and micro-particle, or genetically modified cell. In this review, we will discuss the history and current technologies of drug, protein and gene delivery in the context of regenerative medicine. Next we will present case examples of how delivery technologies are being applied to promote angiogenesis in non-healing wounds or prevent angiogenesis in age related macular degeneration. Finally, we will conclude with a brief discussion of the regulatory pathway from bench-to-bedside for the clinical translation of these novel therapeutics.
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