Investigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows.
Investigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows.
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DOI：
10.3390/ani11113108
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发表时间：
2021-10-30
期刊：
影响因子：
--
通讯作者：
Chen MJ
中科院分区：
文献类型：
--
作者：
Chuang ST;Li KY;Tu PW;Ho ST;Hsu CC;Hsieh JC;Chen MJ
奶牛乳房炎是一种炎症性疾病，通常由细菌感染引起。在本研究中，我们确定了奶牛乳房炎的瘤胃微生物标志物和代谢产物。对瘤胃微生物区系、代谢组和乳房炎之间相互关系的研究表明，健康奶牛的短链脂肪酸(SCFA)产生菌群和与抗炎和抗菌活性相关的代谢物显著高于乳房炎奶牛。已确定的潜在物种和代谢物可能为未来针对瘤胃微生物区系预防/治疗乳房炎提供一个新的视角。奶牛乳房炎严重影响奶牛的生产性能，最终降低了奶牛的盈利能力。奶牛的瘤胃微生物区系、代谢组和乳房炎与早期炎症因子(血清促炎细胞因子)之间的相互关系尚未被探索，因此，本研究评估了荷斯坦奶牛早期瘤胃微生物区系、代谢组和乳房炎之间的相互关系，以确定潜在的微生物生物标志物及其与瘤胃代谢产物的关系。对8头健康奶牛和8头乳房炎奶牛的瘤胃液进行了微生物区系和代谢物分析。分别用线性判别分析效应大小(LEFSE)算法和Spearman相关分析确定与体细胞计数(SCC)和血清促炎细胞因子相关的瘤胃微生物标志物和代谢产物。SCC水平和促炎细胞因子水平与Sharpea呈正相关，与反刍球菌科UCG-014、黄色瘤胃球菌和嗜糖密螺旋体呈负相关。此外，代谢产物黄原酸和1-(1H-苯并[d]咪唑-2-基)乙烷-1-醇与健康奶牛的微生物标志物呈正相关，而黄嘌呤、泛酸和腰果酸与乳房炎奶牛的微生物标志物呈负相关。综上所述，黄色瘤胃球菌和嗜糖密螺旋体是改善奶牛健康的潜在菌株。本研究为未来针对瘤胃微生物区系的炎症性疾病的防治策略提供了一个新的视角。
Dairy cow mastitis is an inflammatory disease often caused by bacterial infections. In the present study, we identified the ruminal microbial biomarkers and metabolites of mastitis in dairy cows. The investigation of the reciprocal interrelationships among the ruminal microbiota, metabolome, and mastitis revealed that short-chain fatty acid (SCFA)-producing microflora and the metabolites related to anti-inflammation and antibacterial activity were significantly higher in healthy cows than in those with mastitis. The identified potential species and metabolites might provide a novel perspective to assist in targeting the ruminal microbiota with preventive/therapeutic strategies against mastitis in the future. Mastitis in dairy cow significantly affects animal performance, ultimately reducing profitability. The reciprocal interrelationships among ruminal microbiota, metabolome, and mastitis combining early inflammatory factors (serum proinflammatory cytokines) in lactating dairy cows has not been explored, thus, this study evaluated these reciprocal interrelationships in early lactating Holstein dairy cows to identify potential microbial biomarkers and their relationship with ruminal metabolites. The ruminal fluid was sampled from 8 healthy and 8 mastitis cows for the microbiota and metabolite analyses. The critical ruminal microbial biomarkers and metabolites related to somatic cell counts (SCC) and serum proinflammatory cytokines were identified by the linear discriminant analysis effect size (LEfSe) algorithm and Spearman’s correlation analysis, respectively. The SCC level and proinflammatory cytokines positively correlated with Sharpea and negatively correlated with Ruminococcaceae UCG-014, Ruminococcus flavefaciens, and Treponema saccharophilum. Furthermore, the metabolites xanthurenic acid, and 1-(1H-benzo[d]imidazol-2-yl) ethan-1-ol positively correlated with microbial biomarkers of healthy cows, whereas, xanthine, pantothenic acid, and anacardic acid were negatively correlated with the microbial biomarkers of mastitis cows. In conclusion, Ruminococcus flavefaciens and Treponema saccharophilum are potential strains for improving the health of dairy cows. The current study provides a novel perspective to assist in targeting the ruminal microbiota with preventive/therapeutic strategies against inflammatory diseases in the future.
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