Genomic surveillance of Acinetobacter baumannii in the Philippines, 2013-2014.
Genomic surveillance of Acinetobacter baumannii in the Philippines, 2013-2014.
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DOI：
10.5365/wpsar.2021.12.4.863
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Philippines Antimicrobial Resistance Surveillance Program
中科院分区：
文献类型：
--
作者：
Chilam J;Argimón S;Limas MT;Masim ML;Gayeta JM;Lagrada ML;Olorosa AM;Cohen V;Hernandez LT;Jeffrey B;Abudahab K;Hufano CM;Sia SB;Holden MTG;Stelling J;Aanensen DM;Carlos CC;Philippines Antimicrobial Resistance Surveillance Program
鲍曼不动杆菌是一种机会性医院病原体，在全世界范围内对碳青霉烯类抗生素的耐药性日益增强。在菲律宾，碳青霉烯类耐药率和多重耐药率均超过50%。我们对菲律宾的碳青霉烯类耐药鲍曼不动杆菌进行了基因组研究，以表征种群多样性和抗菌素耐药机制。我们对 2013 年至 2014 年间菲律宾 16 家医院回收的 117 个鲍曼不动杆菌分离株的全基因组进行了测序。从基因组序列中，我们确定了多位点序列类型、获得性抗生素耐药性决定因素的存在以及分离株之间的相关性。我们还比较了表型和基因型抗性结果。碳青霉烯类耐药性主要是通过 D 类 β-内酰胺酶基因 blaOXA-23 的获得来解释的。亚胺培南表型和基因型耐药性的一致性为 98.15%，分析的 7 种抗生素的总体一致性为 94.97%。鉴定出 22 种不同的序列类型，其中包括 7 种新类型。该群体以高风险国际克隆 2（即克隆复合体 92）为主，特别是 ST195 和 ST208 及其单基因座变体。使用全基因组测序，我们确定了代表潜在未被发现的医院暴发的局部集群，以及表明医院间传播的多医院集群。与全球基因组的比较表明，菲律宾出现碳青霉烯类抗药性国际克隆2可能是克隆扩张和地理传播的结果，至少部分原因是医院感染控制和预防不足。这是菲律宾首次对耐碳青霉烯鲍曼不动杆菌进行广泛的基因组研究，它强调了医院感染控制和预防措施以遏制高风险克隆的重要性。
Acinetobacter baumannii is an opportunistic nosocomial pathogen that has increasingly become resistant to carbapenems worldwide. In the Philippines, rates of carbapenem resistance and multidrug resistance are above 50%. We undertook a genomic study of carbapenem-resistant A. baumannii in the Philippines to characterize the population diversity and antimicrobial resistance mechanisms. We sequenced the whole genomes of 117 A. baumannii isolates recovered by 16 hospitals in the Philippines between 2013 and 2014. From the genome sequences, we determined the multilocus sequence type, presence of acquired determinants of antimicrobial resistance and relatedness between isolates. We also compared the phenotypic and genotypic resistance results. Carbapenem resistance was mainly explained by acquisition of the class-D β-lactamase gene blaOXA-23. The concordance between phenotypic and genotypic resistance to imipenem was 98.15%, and it was 94.97% overall for the seven antibiotics analysed. Twenty-two different sequence types were identified, including 7 novel types. The population was dominated by the high-risk international clone 2 (i.e. clonal complex 92), in particular by ST195 and ST208 and their single locus variants. Using whole-genome sequencing, we identified local clusters representing potentially undetected nosocomial outbreaks, as well as multihospital clusters that indicated interhospital dissemination. Comparison with global genomes suggested that the establishment of carbapenem-resistant international clone 2 in the Philippines is likely the result of clonal expansion and geographical dissemination, and at least partly explained by inadequate hospital infection control and prevention. This is the first extensive genomic study of carbapenem-resistant A. baumannii in the Philippines, and it underscores the importance of hospital infection control and prevention measures to contain high-risk clones.
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影响因子：
4.4
作者：
Hawkey J;Hamidian M;Wick RR;Edwards DJ;Billman-Jacobe H;Hall RM;Holt KE
通讯作者：
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影响因子：
--
作者：
Jolley KA;Bray JE;Maiden MCJ
通讯作者：
Maiden MCJ
影响因子：
5.2
作者：
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影响因子：
4.9
作者：
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通讯作者：
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DOI：
10.1016/j.ijantimicag.2012.01.002
发表时间：
2012-04-01
影响因子：
10.8
作者：
Kiratisin, Pattarachai;Chongthaleong, Anan;Davies, Todd
通讯作者：
Davies, Todd