A methodology for preparing nanostructured biomolecular interfaces with high enzymatic activity.
A methodology for preparing nanostructured biomolecular interfaces with high enzymatic activity.
复制标题
一种制备具有高酶活性的纳米结构生物分子界面的方法。
作者：
Wong LS
The development of a novel method for functionalizing nanopatterned surfaces with catalytically active proteins is reported. This method involves using dip-pen nanolithography (DPN) and polymer pen lithography (PPL) to generate nanoscale patterns of coenzyme A, followed by a phosphopantetheinyl transferase-mediated coupling between coenzyme A and proteins fused to the ybbR-tag. By exploiting the ability to generate protein features over large areas afforded by DPN and PPL, it was now possible to measure protein activity directly on these surfaces. It was found that proteins immobilized on the nanoscale features not only display higher activity per area with decreasing feature size, but are also robust and can be used for repeated catalytic cycles. The immobilization method is applicable to a variety of proteins and gives rise to superior activity compared to proteins attached in random orientations on the surface.
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DOI：
10.1021/la900799m
发表时间：
2009-12-15
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Fears KP;Latour RA
通讯作者：
Latour RA
由模块化聚乙二醇化二硫化物生成的自组装单分子层上的纳米级生物分子结构。
DOI：
10.1002/chem.200902439
发表时间：
2010
期刊：
Chemistry (Weinheim an der Bergstrasse, Germany)
影响因子：
--
作者：
Wong LS
通讯作者：
Wong LS
改进抗体微阵列的亲和耦合：双 (His)6 标记单框架重组抗体片段的工程
影响因子：
3.4
作者：
C. Steinhauer;C. Wingren;F. Khan;M. He;M. Taussig;C. Borrebaeck
通讯作者：
C. Borrebaeck
用于制造蛋白质纳米阵列的核糖体展示和浸笔纳米光刻
DOI：
--
发表时间：
2008
期刊：
影响因子：
--
作者：
Jung Dong Kim;Dae;Jong;Woojun Park;Hyungil Jung
通讯作者：
Hyungil Jung
影响因子：
16.6
作者：
Zheng, Zijian;Daniel, Weston L.;Giam, Louise R.;Huo, Fengwei;Senesi, Andrew J.;Zheng, Gengfeng;Mirkin, Chad A.
通讯作者：
Mirkin, Chad A.