Multiplexed protein arrays enabled by polymer pen lithography: addressing the inking challenge.
Multiplexed protein arrays enabled by polymer pen lithography: addressing the inking challenge.
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DOI：
10.1002/anie.200902649
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发表时间：
2009
影响因子：
16.6
通讯作者：
Mirkin, Chad A.
中科院分区：
文献类型：
--
作者：
Zheng, Zijian;Daniel, Weston L.;Giam, Louise R.;Huo, Fengwei;Senesi, Andrew J.;Zheng, Gengfeng;Mirkin, Chad A.
以低成本和高通量的方式制造蛋白质微阵列和纳米阵列的能力对于包括药物筛选、材料组装、医学诊断、生物传感器和基础生物学研究在内的各种应用都很重要。[1-3]制备蛋白质微阵列的传统方法包括光刻和喷墨印刷。最近，由于高密度蛋白质阵列可以提供更高的检测灵敏度，并且原则上允许用一个芯片筛选数百万种生物标记物，研究也集中在蛋白质模式小型化到纳米范围蛋白质纳米模式也可以提供对重要的基本生物过程的洞察，如细胞粘附和分化。[6-9]事实上，在单个细胞下放置一组蛋白质甚至多个蛋白质结构的能力为研究细胞与表面之间的多价相互作用提供了机会，并指出了纳米阵列技术的主要能力，而类似的微尺度结构无法提供。在此，我们报告了一种新的和快速的策略，将纳米级探针与不同的蛋白质相连接，这些蛋白质可以通过称为聚合物笔光刻（PPL）的技术转移到表面利用这种方法，我们已经以每秒15万个特征的速度生成了100纳米以下的结构。人们已经探索了许多新技术来实现单组分蛋白质特征的小型化和多种蛋白质的微图案化，包括微接触印刷、[11-14]纳米压印光刻、[15]电子束光刻、[16]和各种扫描探针光刻。[4,17 - 22]迄今为止，仅报道了几个纳米图案化多种蛋白质的例子，其中大多数例子使用
The ability to fabricate protein micro-and nanoarrays in a low-cost and high-throughput manner is important for a wide variety of applications, including drug screening, materials assembly, medical diagnostics, biosensors, and fundamental biological studies.[1–3] Traditional approaches to making protein microarrays include photolithography and inkjet printing. Recently, studies also have focused on the miniaturization of protein patterns into the nanometer regime because high density protein arrays can provide increased detection sensitivity and, in principle, allow one to screen millions of biomarkers with one chip.[4] Protein nanopatterns also can provide insight into important fundamental biological processes,[5] such as cell adhesion and differentiation.[6–9] Indeed, the ability to place an array of proteins or even multiple protein structures underneath a single cell opens up the opportunity to study multivalent interactions between a cell and a surface, and points to a major capability of nanoarray technology not afforded by analogous microscale structures. Herein, we report a novel and rapid strategy for inking nanoscale probes with different proteins, which can be transferred to a surface through the technique known as Polymer Pen Lithography (PPL).[10] Using this approach, we have generated sub-100 nm structures at a rate of 150000 features per second.Many new techniques have been explored for miniaturizing single component protein features and micropatterning of multiple proteins, including microcontact printing,[11–14] nanoimprint lithography,[15] e-beam lithography,[16] and a variety of scanning probe lithographies.[4, 17–22] To date, only a few examples of nanopatterning multiple proteins have been reported, and the majority among these examples uses
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影响因子：
15
作者：
Christman, Karen L.;Schopf, Eric;Broyer, Rebecca M.;Li, Ronald C.;Chen, Yong;Maynard, Heather D.
通讯作者：
Maynard, Heather D.
影响因子：
56.9
作者：
Piner, RD;Zhu, J;Mirkin, CA
通讯作者：
Mirkin, CA
影响因子：
3.9
作者：
Kenseth, JR;Harnisch, JA;Porter, MD
通讯作者：
Porter, MD
影响因子：
56.9
作者：
Lee, KB;Park, SJ;Mrksich, M
通讯作者：
Mrksich, M
影响因子：
16.6
作者：
Coyer, Sean R.;Garcia, Andres J.;Delamarche, Emmanuel
通讯作者：
Delamarche, Emmanuel