Ectopic Expression of CsKCS6 From Navel Orange Promotes the Production of Very-Long-Chain Fatty Acids (VLCFAs) and Increases the Abiotic Stress Tolerance of Arabidopsis thaliana.
Ectopic Expression of CsKCS6 From Navel Orange Promotes the Production of Very-Long-Chain Fatty Acids (VLCFAs) and Increases the Abiotic Stress Tolerance of Arabidopsis thaliana.
复制标题
脐橙中 CsKCS6 的异位表达促进极长链脂肪酸 (VLCFA) 的产生并提高拟南芥的非生物胁迫耐受性
DOI：
10.3389/fpls.2020.564656
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发表时间：
2020
影响因子：
5.6
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Guo W;Wu Q;Yang L;Hu W;Liu D;Liu Y
角质层蜡质与植物对非生物胁迫的抗性密切相关。3-酮酰辅酶A合酶（KCS）催化极长链脂肪酸（VLCFA）蜡前体的生物合成。本研究从纽荷尔脐橙橙子中分离到一个新的KCS家族基因，命名为CsKCS 6。CsKCS 6蛋白具有属于硫解酶样超家族的两个主要结构域，FAE 1-CUT 1-RppA和ACP_syn_III_C结构域，其分别存在于氨基酸位置80-368和384-466。CsKCS 6在所有组织中都有表达，其中在柱头中表达量最高;此外，CsKCS 6的转录水平也会随着干旱胁迫、盐胁迫和脱落酸（阿坝）处理而发生变化。异源表达CsKCS 6显著增加了拟南芥茎和叶表皮蜡质中VLCFAs的含量，但对总蜡质含量没有显著影响。与野生型（WT）植株相比，转基因植株的叶片透性降低。进一步的研究表明，与WT植物相比，转基因系在脱水胁迫后水分损失和离子渗漏较少，在干旱胁迫处理下存活率增加，在盐胁迫处理下根长和存活率显着增加。我们的研究结果表明，CsKCS 6不仅在合成脂肪酸前体参与蜡合成中发挥重要作用，而且还提高了转基因拟南芥植物对非生物胁迫的耐受性。因此，CsKSC 6的鉴定有助于提高柑橘的抗逆能力。
Cuticular wax is closely related to plant resistance to abiotic stress. 3-Ketoacyl-CoA synthase (KCS) catalyzes the biosynthesis of very-long-chain fatty acid (VLCFA) wax precursors. In this study, a novel KCS family gene was isolated from Newhall navel orange and subsequently named CsKCS6. The CsKCS6 protein has two main domains that belong to the thiolase-like superfamily, the FAE1-CUT1-RppA and ACP_syn_III_C domains, which exist at amino acid positions 80–368 and 384–466, respectively. CsKCS6 was expressed in all tissues, with the highest expression detected in the stigma; in addition, the transcription of CsKCS6 was changed in response to drought stress, salt stress and abscisic acid (ABA) treatment. Heterologous expression of CsKCS6 in Arabidopsis significantly increased the amount of VLCFAs in the cuticular wax on the stems and leaves, but there were no significant changes in total wax content. Compared with that of the wild-type (WT) plants, the leaf permeability of the transgenic plants was lower. Further research showed that, compared with the WT plants, the transgenic lines experienced less water loss and ion leakage after dehydration stress, displayed increased survival under drought stress treatment and presented significantly longer root lengths and survival under salt stress treatment. Our results indicate that CsKCS6 not only plays an important role in the synthesis of fatty acid precursors involved in wax synthesis but also enhances the tolerance of transgenic Arabidopsis plants to abiotic stress. Thus, the identification of CsKSC6 could help to increase the abiotic stress tolerance of Citrus in future breeding programs.
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影响因子：
11.6
作者：
Fiebig, A;Mayfield, JA;Preuss, D
通讯作者：
Preuss, D
影响因子：
7.4
作者：
JENKS, MA;JOLY, RJ;ASHWORTH, EN
通讯作者：
ASHWORTH, EN
影响因子：
5.1
作者：
Liu, Dechun;Yang, Li;Liu, Yong
通讯作者：
Liu, Yong
影响因子：
4.9
作者：
Harada, Emiko;Kim, Ji-Ah;Choi, Yong-Eui
通讯作者：
Choi, Yong-Eui
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影响因子：
3.1
作者：
Guo, Hai-Song;Zhang, Yan-Mei;Xue, Jia-Yu
通讯作者：
Xue, Jia-Yu