Transcriptional analysis of the effect of exogenous decanoic acid stress on Streptomyces roseosporus.
Transcriptional analysis of the effect of exogenous decanoic acid stress on Streptomyces roseosporus.
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外源癸酸胁迫对玫瑰孢链霉菌影响的转录分析
DOI：
10.1186/1475-2859-12-19
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发表时间：
2013-02-21
影响因子：
6.4
通讯作者：
Xie J
中科院分区：
文献类型：
--
作者：
Liao G;Liu Q;Xie J
Backgroud
Daptomycin is an important antibiotic against infections caused by drug-resistant pathogens. Its production critically depends on the addition of decanoic acid during fermentation. Unfortunately, decanoic acid (2.5 mM) is toxic to daptomycin producer, Streptomyces roseosporus.
Results
To understand the mechanism underlying decanoic tolerance or toxicity, the responses of S. roseosporus was determined by a combination of phospholipid fatty acid analysis, reactive oxygen species (ROS) measurement and RNA sequencing. Assays using fluorescent dyes indicated a sharp increase in reactive oxygen species during decanoic acid stress; fatty acid analysis revealed a marked increase in the composition of branched-chain fatty acids by approximately 10%, with a corresponding decrease in straight-chain fatty acids; functional analysis indicated decanoic acid stress has components common to other stress response, including perturbation of respiratory functions (nuo and cyd operons), oxidative stress, and heat shock. Interestingly, our transcriptomic analysis revealed that genes coding for components of proteasome and related to treholase synthesis were up-regulated in the decanoic acid –treated cells.
Conclusion
These findings represent an important first step in understanding mechanism of decanoic acid toxicity and provide a basis for engineering microbial tolerance.
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通过记者引导的 rpsL 突变选择提高玫瑰孢链霉菌中 A21978C 的产量
影响因子：
4
作者：
Wang, L.;Zhao, Y.;Liao, G.
通讯作者：
Liao, G.
影响因子：
3
作者：
Lu, Wenyu;Fan, Jinghua;Caiyin, Qinggele
通讯作者：
Caiyin, Qinggele
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
4.1
作者：
HUBER, FM;PIEPER, RL;TIETZ, AJ
通讯作者：
TIETZ, AJ
影响因子：
56.9
作者：
Darwin, KH;Ehrt, S;Nathan, CF
通讯作者：
Nathan, CF