The RhoGEF protein Plekhg5 regulates medioapical and junctional actomyosin dynamics of apical constriction during Xenopus gastrulation.
The RhoGEF protein Plekhg5 regulates medioapical and junctional actomyosin dynamics of apical constriction during Xenopus gastrulation.
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DOI：
10.1091/mbc.e22-09-0411
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发表时间：
2023-06-01
影响因子：
3.3
通讯作者：
Chang, Chenbei
中科院分区：
文献类型：
--
作者：
Baldwin, Austin;Popov, Ivan K.;Keller, Ray;Wallingford, John;Chang, Chenbei
顶端收缩导致上皮细胞的顶端表面减少，并且是上皮形态发生的广泛使用的机制。中间顶端和交界处的肌动球蛋白重塑都参与了顶端收缩，但中间与交界处的肌动球蛋白的部署及其在脊椎动物胚胎发育中的遗传调控尚未得到充分描述。在这项研究中，我们调查肌动球蛋白动力学和他们的调节RhoGEF蛋白Plekhg 5在非洲爪蟾瓶细胞。使用实时成像和定量图像分析，我们表明，瓶细胞呈现不同的形状，与圆形瓶细胞收缩早期在小集群，然后梭形瓶细胞之间形成的集群。尽管根尖收缩与根尖肌动球蛋白定位的相关性似乎更强，但随着表面积的减小，根尖和交界处肌动球蛋白信号都增加。F-肌动蛋白束垂直于顶端表面形成收缩的细胞，这可能对应于微绒毛先前观察到的顶膜。击倒plekhg 5破坏medioapical和交界肌动球蛋白活性和顶端收缩，但不影响初始F-肌动蛋白动力学。将这些结果结合在一起，我们的研究揭示了不同的细胞形态，揭示了肌动球蛋白的行为，并证明了RhoGEF蛋白在控制肌动球蛋白动力学在非洲爪蟾原肠胚瓶细胞顶端收缩过程中的关键作用。
Apical constriction results in apical surface reduction in epithelial cells and is a widely used mechanism for epithelial morphogenesis. Both medioapical and junctional actomyosin remodeling are involved in apical constriction, but the deployment of medial versus junctional actomyosin and their genetic regulation in vertebrate embryonic development have not been fully described. In this study, we investigate actomyosin dynamics and their regulation by the RhoGEF protein Plekhg5 in Xenopus bottle cells. Using live imaging and quantitative image analysis, we show that bottle cells assume different shapes, with rounding bottle cells constricting earlier in small clusters followed by fusiform bottle cells forming between the clusters. Both medioapical and junctional actomyosin signals increase as surface area decreases, though correlation of apical constriction with medioapical actomyosin localization appears to be stronger. F-actin bundles perpendicular to the apical surface form in constricted cells, which may correspond to microvilli previously observed in the apical membrane. Knockdown of plekhg5 disrupts medioapical and junctional actomyosin activity and apical constriction but does not affect initial F-actin dynamics. Taking the results together, our study reveals distinct cell morphologies, uncovers actomyosin behaviors, and demonstrates the crucial role of a RhoGEF protein in controlling actomyosin dynamics during apical constriction of bottle cells in Xenopus gastrulation.
影响因子：
2.7
作者：
Baldwin, Austin T.;Kim, Juliana H.;Wallingford, John B.
通讯作者：
Wallingford, John B.
影响因子：
2.5
作者：
Lee, Chanjae;Le, Minh-Phuong;Wallingford, John B.
通讯作者：
Wallingford, John B.