Identification and characterization of heat shock 70 genes in Aedes aegypti (Diptera: Culicidae).
Identification and characterization of heat shock 70 genes in Aedes aegypti (Diptera: Culicidae).
复制标题
DOI：
10.1603/033.046.0313
复制
发表时间：
2009-05
影响因子：
2.1
通讯作者：
Adelman ZN
中科院分区：
文献类型：
--
作者：
Gross TL;Myles KM;Adelman ZN
热休克基因在进化上高度保守，在所有生物体中响应于应激而不同程度地表达。热休克70（hsp 70）基因已被很好地描述了一些生物体，最显着的果蝇，但尚未为任何主要的节肢动物传播的病毒蚊子载体。为了鉴定埃及伊蚊（双翅目：蚊科）中的热休克蛋白70基因，对埃及伊蚊的热休克蛋白70基因进行了基本的局部比对搜索。埃及伊蚊的基因组，使用D.黑腹果蝇Hsp 70蛋白序列作为查询。两个簇的六个以前未注释的AaHsp 70基因被确定，并发现被组织成三对几乎相同的开放阅读框架，映射到两个基因组支架。与热休克基因的命名一致，在正常饲养条件（28°C）下没有观察到可检测水平的AaHsp 70基因表达，在37-39°C的热休克下观察到稳健的表达。北方分析表明，热诱导表达的推定AaHsp 70基因在所有的生命阶段，并在所有组织中测试的时间和温度依赖性的方式。野外捕获的Ae.埃及人可以作为压力的一般标志。此外，来自AaHsp 70基因的启动子序列可用于以诱导方式控制转基因的表达。
Heat shock genes are highly evolutionarily conserved and are expressed to varying degrees in all organisms in response to stress. Heat shock 70 (hsp70) genes have been well characterized in a number of organisms, most notably Drosophila melanogaster, but not as yet for any of the major arthropod-borne viral mosquito vectors. To identify hsp70 genes in the yellow fever mosquito, Aedes aegypti (Diptera: Culicidae), basic local alignment searches of the Ae. aegypti genome were performed using D. melanogaster Hsp70 protein sequences as query. Two clusters of six previously unannotated AaHsp70 genes were identified and found to be organized into three pairs of nearly identical open reading frames, which mapped to two genomic scaffolds. Consistent with a designation as heat shock genes, no detectable level of expression of AaHsp70 genes was observed under normal rearing conditions (28°C), with robust expression observed with a heat shock of 37–39°C. Northern analysis showed heat-inducible expression of putative AaHsp70 genes at all life stages and in all tissues tested in a time- and temperature-dependent manner. Monitoring of AaHsp70 gene expression levels in field-caught Ae. aegypti may serve as a general marker for stress. In addition, promoter sequences from AaHsp70 genes may be used to control the expression of transgenes in an inducible manner.
登录
查看更多内容
影响因子：
2.6
作者：
Benedict, M.Q.;Cockburn, A.F.;Seawright, J.A.
通讯作者：
Seawright, J.A.
影响因子：
2.4
作者：
Uhlirov치, M;Asahina, M;Jindra, M
通讯作者：
Jindra, M
DOI：
10.1073/pnas.79.10.3218
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LI, GC;WERB, Z
通讯作者：
WERB, Z
影响因子：
10.7
作者：
Bettencourt, BR;Feder, ME
通讯作者：
Feder, ME
影响因子：
1.6
作者：
Evgen'ev, MB;Zatsepina, OG;Feder, ME
通讯作者：
Feder, ME