Airway epithelial cell migration dynamics. MMP-9 role in cell-extracellular matrix remodeling.
Airway epithelial cell migration dynamics. MMP-9 role in cell-extracellular matrix remodeling.
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DOI：
10.1083/jcb.146.2.517
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发表时间：
1999-07-26
期刊：
影响因子：
--
通讯作者：
Tournier JM
中科院分区：
文献类型：
--
作者：
Legrand C;Gilles C;Zahm JM;Polette M;Buisson AC;Kaplan H;Birembaut P;Tournier JM
与92-kD明胶酶（基质金属蛋白酶9或MMP-9）的表达相关的细胞铺展和迁移是参与呼吸道上皮修复的重要机制。我们研究了MMP-9的定位及其在迁移的人支气管上皮细胞（HBEC）中的潜在作用。在体内和体外实验中，MMP-9在位于伤口前缘的迁移HBEC中积累，并且MMP-9表达抑制细胞迁移速度。MMP-9通过肌动蛋白依赖性途径在迁移细胞的推进板状伪足中积累，随后在细胞外基质（ECM）中被发现具有活性。板状伪足通过与IV型胶原建立的原始接触而锚定。MMP-9聚集在IV型胶原蛋白后面，细胞-ECM接触较少。胶原蛋白IV和MMP-9都参与细胞迁移，因为当细胞-胶原蛋白IV相互作用被阻断时，细胞轻微扩散但不迁移;当MMP-9活化被阻止时，细胞保持固定在原始接触上，根本不前进。这些观察结果表明，MMP-9通过重塑与原始接触有关的临时ECM来控制修复HBEC的迁移。
Cell spreading and migration associated with the expression of the 92-kD gelatinase (matrix metalloproteinase 9 or MMP-9) are important mechanisms involved in the repair of the respiratory epithelium. We investigated the location of MMP-9 and its potential role in migrating human bronchial epithelial cells (HBEC). In vivo and in vitro, MMP-9 accumulated in migrating HBEC located at the leading edge of a wound and MMP-9 expression paralleled cell migration speed. MMP-9 accumulated through an actin-dependent pathway in the advancing lamellipodia of migrating cells and was subsequently found active in the extracellular matrix (ECM). Lamellipodia became anchored through primordial contacts established with type IV collagen. MMP-9 became amassed behind collagen IV where there were fewer cell–ECM contacts. Both collagen IV and MMP-9 were involved in cell migration because when cell–collagen IV interaction was blocked, cells spread slightly but did not migrate; and when MMP-9 activation was prevented, cells remained fixed on primordial contacts and did not advance at all. These observations suggest that MMP-9 controls the migration of repairing HBEC by remodeling the provisional ECM implicated in primordial contacts.
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影响因子：
3.6
作者：
ERJEFALT, JS;ERJEFALT, I;PERSSON, CGA
通讯作者：
PERSSON, CGA
影响因子：
20.1
作者：
BENDECK, MP;ZEMPO, N;REIDY, MA
通讯作者：
REIDY, MA
DOI：
10.1165/ajrcmb.17.4.2877
发表时间：
1997-10-01
影响因子：
6.4
作者：
Okada, S;Kita, H;Leiferman, KM
通讯作者：
Leiferman, KM
影响因子：
19
作者：
Lee, Juliet;Ishihara, Akira;Jacobson, Ken
通讯作者：
Jacobson, Ken
影响因子：
4
作者：
Kelly, T;Yan, Y;Chu, WS
通讯作者：
Chu, WS