Effect of endurance exercise on microRNAs in myositis skeletal muscle-A randomized controlled study.
Effect of endurance exercise on microRNAs in myositis skeletal muscle-A randomized controlled study.
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耐力运动对肌炎骨骼肌肌肉A随机对照研究的影响。
DOI：
10.1371/journal.pone.0183292
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Nagaraju K
中科院分区：
文献类型：
--
作者：
Boehler JF;Hogarth MW;Barberio MD;Novak JS;Ghimbovschi S;Brown KJ;Alemo Munters L;Loell I;Chen YW;Gordish-Dressman H;Alexanderson H;Lundberg IE;Nagaraju K
鉴定耐力运动后骨骼肌 microRNA 表达的变化，并将鉴定的 microRNA 与 mRNA 和蛋白质表达与多发性肌炎 (PM) 和皮肌炎 (DM) 患者的疾病特异性途径相关联。在一项平行临床试验设计之后，卡罗林斯卡大学医院将疑似 PM 或 DM、每周锻炼少于一次、并接受至少一个月稳定药物治疗的患者随机分为两组：12 周耐力运动组 (n = 12) 或不运动对照组 (n = 11)。使用 Affymetrix 微阵列，测定每组 3 名患者运动干预前后的配对肌肉活检中的 microRNA 表达。使用 microRNA 目标过滤器进行 Ingenuity 通路分析来识别 microRNA 转录目标。这些靶标在患者的 mRNA（微阵列）和蛋白质（质谱）水平上进行了研究。耐力运动改变了 39 个 microRNA。表达增加的 microRNA 预计会靶向参与炎症过程、新陈代谢和肌肉萎缩的转录本。此外，这些目标转录本与运动患者的 mRNA 表达减少有关。特别是，NF-κB 调节因子 IKBKB 的减少与其靶标 microRNA (miR-196b) 的增加相关。在蛋白质水平上，线粒体蛋白质（AK3、HIBADH）增加，这与预计调节其表达的 microRNA 减少有关。运动后疾病表型的改善与在转录水平上靶向和下调免疫过程的 microRNA 增加以及在蛋白质水平上靶向和上调线粒体含量的 microRNA 减少有关。因此，microRNA 可以通过减少免疫反应和增加线粒体生物发生来改善疾病。临床试验.gov NCT01184625
To identify changes in skeletal muscle microRNA expression after endurance exercise and associate the identified microRNAs with mRNA and protein expression to disease-specific pathways in polymyositis (PM) and dermatomyositis (DM) patients. Following a parallel clinical trial design, patients with probable PM or DM, exercising less than once a week, and on stable medication for at least one month were randomized into two groups at Karolinska University Hospital: a 12-week endurance exercise group (n = 12) or a non-exercised control group (n = 11). Using an Affymetrix microarray, microRNA expression was determined in paired muscle biopsies taken before and after the exercise intervention from 3 patients in each group. Ingenuity pathway analysis with a microRNA target filter was used to identify microRNA transcript targets. These targets were investigated at the mRNA (microarray) and protein (mass spectrometry) levels in patients. Endurance exercise altered 39 microRNAs. The microRNAs with increased expression were predicted to target transcripts involved in inflammatory processes, metabolism, and muscle atrophy. Further, these target transcripts had an associated decrease in mRNA expression in exercised patients. In particular, a decrease in the NF-κB regulator IKBKB was associated with an increase in its target microRNA (miR-196b). At the protein level, there was an increase in mitochondrial proteins (AK3, HIBADH), which were associated with a decrease in microRNAs that were predicted to regulate their expression. Improvement in disease phenotype after exercise is associated with increasing microRNAs that target and downregulate immune processes at the transcript level, as well as decreasing microRNAs that target and upregulate mitochondrial content at the protein level. Therefore, microRNAs may improve disease by decreasing immune responses and increasing mitochondrial biogenesis. ClinicalTrials.gov NCT01184625
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影响因子：
3.4
作者：
Holloway, Kathryn V.;O'Gorman, Martin;Burniston, Jatin G.
通讯作者：
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Harris-Love, M. O.;Shrader, J. A.;Koziol, D.;Pahlajani, N.;Jain, M.;Smith, M.;Cintas, H. L.;McGarvey, C. L.;James-Newton, L.;Pokrovnichka, A.;Moini, B.;Cabalar, I.;Lovell, D. J.;Wesley, R.;Plotz, P. H.;Miller, F. W.;Hicks, J. E.;Rider, L. G.
通讯作者：
Rider, L. G.
影响因子：
4.9
作者：
Coley W;Rayavarapu S;Nagaraju K
通讯作者：
Nagaraju K