The cloning of growth associated protein 43 of Gekko japonicus and its effect on cell morphology
The cloning of growth associated protein 43 of Gekko japonicus and its effect on cell morphology
复制标题
壁虎生长相关蛋白43的克隆及其对细胞形态的影响
DOI：
10.1007/s11033-012-1616-x
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发表时间：
2012-03
影响因子：
2.8
通讯作者：
Liu, Yan
中科院分区：
文献类型：
--
作者：
Feng, Xiao;Zhou, Youlang;Liu, Mei;Gu, Xingxing;Wang, Yongjun;Ding, Fei;Gu, Xiaosong;Liu, Yan
从大壁虎脑和脊髓cDNA文库中克隆了大壁虎生长相关蛋白43（GAP-43）基因。北方杂交结果表明，壁虎GAP-43基因的转录本长度为1.7 kb，在脑、脊髓和卵巢组织中有丰富的表达。Gecko GAP-43在体外可促进Gsn 3细胞和PC 12细胞的生长，丝氨酸42位磷酸化可调节GAP-43对细胞铺展和形态的影响。采用逆转录聚合酶链反应（RT-PCR）检测断尾后脊髓GAP-43表达的变化。在我们检查的时间过程中，脊髓中GAP-43的水平增加，表明GAP-43表达与脊髓损伤和再生之间可能存在相关性。
The growth-associated protein 43 (GAP-43) gene of Gekko japonicus was obtained from a brain and spinal cord cDNA library. The results of northern blot analysis showed the gecko GAP-43 gene transcript is 1.7 kb in length, and it was abundantly expressed in tissues of brain, spinal cord and ovary. Gecko GAP-43 promoted the outgrowth of Gsn3 cells and PC12 cell in vitro, and phosphorylation at serine 42 modulated the effect of GAP-43 on cell spreading and morphology. The change in GAP-43 expression in the spinal cord after tail amputation was examined by reverse transcription polymerase chain reaction (RT-PCR). The level of GAP-43 in the spinal cord was increased during the time course we examined, indicating a possible correlation between GAP-43 expression and the spinal cord injury and regeneration.
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影响因子：
4
作者：
Bolsover, SR
通讯作者：
Bolsover, SR
影响因子：
4.3
作者：
Rashmi Mishra;Shailesh Kumar Gupta;K. Meiri;Megin Fong;P. Thostrup;D. Juncker;S. Mani
通讯作者：
Rashmi Mishra;Shailesh Kumar Gupta;K. Meiri;Megin Fong;P. Thostrup;D. Juncker;S. Mani
影响因子：
2.9
作者：
Kaneda, Manabu;Nagashima, Mikiko;Kato, Satoru
通讯作者：
Kato, Satoru
DOI：
10.1016/s0021-9258(18)54708-6
发表时间：
1991-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Tavaré;B. Zhang;L. Ellis;R. Roth
通讯作者：
J. Tavaré;B. Zhang;L. Ellis;R. Roth
影响因子：
3.5
作者：
Nguyen, Lilly;He, Qin;Meiri, Karina F.
通讯作者：
Meiri, Karina F.