The First Whole Genome Sequencing of Sanghuangporus sanghuang Provides Insights into Its Medicinal Application and Evolution.
The First Whole Genome Sequencing of Sanghuangporus sanghuang Provides Insights into Its Medicinal Application and Evolution.
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DOI：
10.3390/jof7100787
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发表时间：
2021-09-22
期刊：
影响因子：
--
通讯作者：
Zhou LW
中科院分区：
文献类型：
--
作者：
Jiang JH;Wu SH;Zhou LW
桑黄孢菌（Sanghuangporus）是一个大型药用真菌属，以桑黄孢菌（S.桑黄，中国古代人在传统中药中使用的物种。为方便S.桑黄，我们首次从单核菌株中进行了它的基因组测序分析。将33.34 Mb的基因组序列组装成26个重叠群，这导致8278个蛋白质编码基因的预测。从这些基因中首次鉴定出桑黄孔菌中除三萜类化合物外，还可能存在倍半萜类化合物的生物合成途径。多糖类物质是S.与其它大型药用真菌类群相比，桑黄、黄酮类化合物是含量特别丰富的药用代谢产物。从基因组的角度来看，S.桑黄具有四极异宗配合系统，与S. baumii和S.香草味的。基因组学分析表明，桑黄孔菌出现于1539万年前，S. sanghuang与S. baumii比S.香草味的。然而，S.桑黄与S. vaninii比S.鲍姆与S. baumii和S. vaninii的研究结果表明，桑黄孢菌的物种多样性可能是由基因组序列的易位和易位加倒位驱动的，而基因家族的扩张和收缩可能是桑黄孢菌物种寄主特异性的原因。总的来说，S.《桑黄》介绍了它的药用和演变。
Sanghuangporus is a medicinal macrofungal genus typified by S. sanghuang, the very species utilized in traditional Chinese medicines by Chinese ancient people. To facilitate the medicinal application of S. sanghuang, we, for the first time, perform its genome sequencing and analyses from a monokaryon strain. A 33.34 Mb genome sequence was assembled to 26 contigs, which lead to the prediction of 8278 protein-coding genes. From these genes, the potential biosynthesis pathway of sesquiterpenoids was, for the first time, identified from Sanghuangporus, besides that of triterpenoids. While polysaccharides are the main medicinal metabolites in S. sanghuang, flavonoids are especially abundant medicinal metabolites comparing with other medicinal macrofungal groups. From the genomic perspective, S. sanghuang has a tetrapolar heterothallic mating system, and has its special nutritional strategy and advantageous medicinal properties compared with S. baumii and S. vaninii. A phylogenomics analysis indicates that Sanghuangporus emerged 15.39 million years ago and S. sanghuang has a closer phylogenetic relationship with S. baumii than S. vaninii. However, S. sanghuang shares a higher region of synteny and more orthologous genes, including carbohydrate-active enzymes with S. vaninii than S. baumii. A comparative genomics analysis with S. baumii and S. vaninii indicates that species diversification within Sanghuangporus may be driven by the translocation and translocation plus inversion of genome sequences, while the expansion and contraction of gene families may contribute to the host specificity of Sanghuangporus species. In general, the genome sequence of S. sanghuang provides insights into its medicinal application and evolution.
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影响因子：
3.8
作者：
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