Valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using Schizochytrium limacinum and Blakeslea trispora.
Valorification of crude glycerol for pure fractions of docosahexaenoic acid and β-carotene production by using Schizochytrium limacinum and Blakeslea trispora.
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DOI：
10.1186/s12934-018-0945-4
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发表时间：
2018-06-16
影响因子：
6.4
通讯作者：
Vodnar DC
中科院分区：
文献类型：
--
作者：
Bindea M;Rusu B;Rusu A;Trif M;Leopold LF;Dulf F;Vodnar DC
The goal of this research is the investigation of a way to maximize the production of docosahexaenoic acid (DHA) and β-carotene by optimizing the culture conditions of their sources, microalgae Schizochytrium limacinum and fungus Blakeslea trispora respectively, in a fermentation medium. The influencing factors in the fermentation process for producing DHA and β-carotene have proven to be: the concentration of carbon source (different glycerol crude and pure concentrations) for both of them, and in particular temperature for DHA and pH for β-carotene. Testing the effect of these parameters was determined: biomass, DHA and β-carotene concentration. The highest production by S. limacinum was obtained at 25 °C, while using a quantity of 90 g/L of glycerol (crude or pure) as a carbon source. Temperature was the main factor that influenced the biosynthesis of DHA. The quantification of DHA was made by GC–MS chromatography, followed by a purification process, with the end result of DHA in pure phase. The maximum quantities for β-carotene production were obtained with pH 7 and 60 g/L of crude glycerol. The results highlight the possibility of using crude glycerol as a low-cost substrates for growth of microalgae S. limacinum and of fungus B. trispora in order to obtain the crucial molecules: docosahexaenoic acid and β-carotene.
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影响因子：
2
作者：
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通讯作者：
Valenzuela, A
DOI：
10.1016/j.plefa.2014.01.005
发表时间：
2015-01-01
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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