Lactobacillus proteins are associated with the bactericidal activity against E. coli of female genital tract secretions.
Lactobacillus proteins are associated with the bactericidal activity against E. coli of female genital tract secretions.
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DOI：
10.1371/journal.pone.0049506
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Herold BC
中科院分区：
文献类型：
--
作者：
Kalyoussef S;Nieves E;Dinerman E;Carpenter C;Shankar V;Oh J;Burd B;Angeletti RH;Buckheit KW;Fredricks DN;Madan RP;Keller MJ;Herold BC
女性生殖道分泌物对大肠杆菌有杀菌作用。Coli的体外表达。然而，对这一活动有贡献的受试者之间的可变性和分子还没有定义。对99例健康女性宫颈阴道灌洗液(CVL)进行了杀菌活性和免疫介质浓度测定。CVL使大肠杆菌集落数减少了68%[−26,100](中位数[范围])。CVL对实验室和临床分离的大肠埃希菌有活性，但对乳杆菌属无活性。杀菌活性与回收的蛋白质浓度相关(p<；0.001)，但与细胞因子、趋化因子或抗菌肽无关。对4个具有90%抑制活性的CVL和2个具有30%活性的CVL进行了MS/MS蛋白质组学分析。共鉴定出215种蛋白质，其中6种仅在活性样本中发现。其中4个对应于皱纹乳杆菌或jensenii蛋白。此外，简氏乳杆菌的培养上清液对大肠杆菌具有杀菌作用。宿主和共生微生物区系蛋白都有助于粘膜防御。对这些蛋白质的鉴定将有助于制定策略，以维持健康的阴道微生物群，并防止大肠杆菌等病原菌的定植，这些病原菌会增加尿路感染、早产和围产期感染的风险。
Female genital tract secretions are bactericidal for Escherichia (E.) coli ex vivo. However, the intersubject variability and molecules that contribute to this activity have not been defined. The bactericidal activity and concentration of immune mediators in cervicovaginal lavage (CVL) collected from 99 healthy women were determined. CVL reduced the number of E. coli colonies by 68% [−26, 100] (median [range]). CVL were active against laboratory and clinical isolates of E. coli, but were inactive against Lactobacillus species. Bactericidal activity correlated with the concentration of protein recovered (p<0.001), but not with cytokines, chemokines or antimicrobial peptides. Four CVL with>90% inhibitory activity (active) and two with<30% activity were subjected to MS/MS proteomic analysis. 215 proteins were identified and six were found exclusively in active samples. Four of these corresponded to Lactobacillus crispatus or jensenii proteins. Moreover, culture supernatants from Lactobacillus jensenii were bactericidal for E. coli. Both host and commensal microbiota proteins contribute to mucosal defense. Identification of these proteins will facilitate the development of strategies to maintain a healthy vaginal microbiome and prevent colonization with pathogenic bacteria such as E. coli that increase the risk for urinary tract infections, preterm labor and perinatal infection.
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影响因子：
2.1
作者：
Atassi, Fabrice;Servin, Alain L.
通讯作者：
Servin, Alain L.
影响因子：
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作者：
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