Identification of novel avian influenza virus derived CD8+ T-cell epitopes.
Identification of novel avian influenza virus derived CD8+ T-cell epitopes.
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DOI：
10.1371/journal.pone.0031953
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Jansen CA
中科院分区：
文献类型：
--
作者：
Reemers SS;van Haarlem DA;Sijts AJ;Vervelde L;Jansen CA
禽流感病毒(AIV)感染是对人类和家禽的持续威胁。流感病毒特异性CD8+T细胞对同源和异种流感病毒株具有保护作用。与对人类和小鼠的描述相反，对鸡的表位特异性CD8+T细胞的了解有限。因此，我们着手确定AIV特异性CD8+T细胞表位。基于锚点残基的表位预测得到33个候选表位。用低致病性AIV株感染MHC-I近交鸡，分别于感染后5、7、10、14d处死鸡。从肺、脾和血液中分离淋巴细胞，用禽流感病毒特异性多肽或单肽体外刺激，ELISPOT法检测干扰素γ的产生。这导致了12个MHC B12限制性、3个B4限制性和1个B19限制性AIV特异性CD8+T细胞表位的鉴定。综上所述，我们已经为几个近交系鸡种确定了新的禽流感病毒衍生的CD8+T细胞表位。这一知识可用于研究CD8+T细胞在流感自然宿主中对抗AIV感染的作用，并可能对疫苗开发具有重要意义。
Avian influenza virus (AIV) infection is a continuing threat to both humans and poultry. Influenza virus specific CD8+ T cells are associated with protection against homologous and heterologous influenza strains. In contrast to what has been described for humans and mice, knowledge on epitope-specific CD8+ T cells in chickens is limited. Therefore, we set out to identify AIV-specific CD8+ T-cell epitopes. Epitope predictions based on anchor residues resulted in 33 candidate epitopes. MHC I inbred chickens were infected with a low pathogenic AIV strain and sacrificed at 5, 7, 10 and 14 days post infection (dpi). Lymphocytes isolated from lung, spleen and blood were stimulated ex vivo with AIV-specific pooled or individual peptides and the production of IFNγ was determined by ELIspot. This resulted in the identification of 12 MHC B12-restricted, 3 B4-restricted and 1 B19-restricted AIV- specific CD8+ T-cell epitopes. In conclusion, we have identified novel AIV-derived CD8+ T-cell epitopes for several inbred chicken strains. This knowledge can be used to study the role of CD8+ T cells against AIV infection in a natural host for influenza, and may be important for vaccine development.
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影响因子：
15.3
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