Improved in vitro blood compatibility of polycaprolactone nanowire surfaces.
Improved in vitro blood compatibility of polycaprolactone nanowire surfaces.
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DOI：
10.1021/am503508r
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发表时间：
2014-09-24
影响因子：
9.5
通讯作者：
Popat, Ketul C.
中科院分区：
文献类型：
--
作者：
Leszczak, Victoria;Popat, Ketul C.
关键词：
目前有多种聚合物材料用于制备各种血液接触的植入式医疗器械。这些装置包括组织移植物、冠状动脉和血管支架以及骨科植入物。此类材料的血栓形成性质可能会给患者带来严重的并发症，并最终导致功能衰竭。迄今为止，还没有真正的血液相容性生物材料表面。纳米结构表面改善了细胞相互作用，但有关其血液相容性的信息有限。在本研究中，研究了四种不同表面（对照，PCL；纳米线，NW；胶原固定对照，cPCL；胶原固定纳米线，cNW）作为血液接触植入物界面的体外血液相容性。这里给出的结果表明，与对照表面相比，纳米线表面的体外血液相容性增强。尽管白细胞粘附力没有显着差异，但 NW 表面上的血小板粘附力有所下降。扫描电子显微镜图像显示，与 PCL 和 cPCL 表面相比，cNW 表面上的血小板/白细胞复合物减少，并且 NW 表面上没有形成明显的复合物。这些复合物的增加可能导致 PCL 和 cPCL 表面上血小板和白细胞活化的特异性标记物的更高表达。在任何表面上的接触和补体激活均未发现显着差异。此外，NW 表面上的凝血酶抗凝血酶复合物显着减少。在 PCL 表面上发现溶血和纤维蛋白原吸附显着增加，这可能是由其疏水表面引起的。这项工作展示了纳米结构表面改善的血液相容性，将这种特定的纳米结构确定为促进血液接触生物材料长期成功的潜在界面。
There are a multitude of polymeric materials currently utilized to prepare a variety of blood-contacting implantable medical devices. These devices include tissue grafts, coronary artery and vascular stents, and orthopedic implants. The thrombogenic nature of such materials can cause serious complications in patients, and ultimately lead to functional failure. To date, there is no truly hemocompatible biomaterial surface. Nanostructured surfaces improve cellular interactions but there is a limited amount of information regarding their blood compatibility. In this study, the in vitro blood compatibility of four different surfaces (control, PCL; nanowire, NW; collagen immobilized control, cPCL; collagen immobilized nanowire, cNW) were investigated for their use as interfaces for blood-contacting implants. The results presented here indicate enhanced in vitro blood compatibility of nanowire surfaces compared control surfaces. Although there were no significant differences in leukocyte adhesion, there was a decrease in platelet adhesion on NW surfaces. Scanning electron microscopy images showed a decrease in platelet/leukocyte complexes on cNW surfaces and no apparent complexes were formed on NW surfaces compared to PCL and cPCL surfaces. The increase in these complexes likely contributed to a higher expression of specific markers for platelet and leukocyte activation on PCL and cPCL surfaces. No significant differences were found in contact and complement activation on any surface. Further, thrombin antithrombin complexes were significantly reduced on NW surfaces. A significant increase in hemolysis and fibrinogen adsorption was identified on PCL surfaces likely caused by its hydrophobic surface. This work shows the improved blood-compatibility of nanostructured surfaces, identifying this specific nanoarchitecture as a potential interface for promoting the long-term success of blood-contacting biomaterials.
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影响因子：
4.8
作者：
Leszczak V;Baskett DA;Popat KC
通讯作者：
Popat KC
DOI：
10.1039/df9480300011
发表时间：
1948-01-01
期刊：
DISCUSSIONS OF THE FARADAY SOCIETY
影响因子：
--
作者：
CASSIE, ABD
通讯作者：
CASSIE, ABD
肝素改性 ePTFE 血管移植物的血液和血管细胞相容性。
影响因子：
14
作者：
Hoshi, Ryan A.;Van Lith, Robert;Jen, Michele C.;Allen, Josephine B.;Lapidos, Karen A.;Ameer, Guillermo
通讯作者：
Ameer, Guillermo
影响因子：
14
作者：
Karlsson, M;Pålsgård, E;Di Silvio, L
通讯作者：
Di Silvio, L
影响因子：
6.7
作者：
Blezer, R;Willems, GM;Lindhout, T
通讯作者：
Lindhout, T