In vivo DNase I sensitivity of the Streptomyces coelicolor chromosome correlates with gene expression: implications for bacterial chromosome structure.
In vivo DNase I sensitivity of the Streptomyces coelicolor chromosome correlates with gene expression: implications for bacterial chromosome structure.
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DOI：
10.1093/nar/gkl649
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发表时间：
2006
影响因子：
14.9
通讯作者：
Bibb, Mervyn
中科院分区：
文献类型：
--
作者：
McArthur, Michael;Bibb, Mervyn
对于细菌，天蓝色链霉菌A3（2）含有相对较大的基因组（8.7 Mb），具有复杂和适应性的基因调控模式。我们发现天蓝色链霉菌基因组的物理结构与其中基因的转录活性之间存在相关性。12个基因进行了调查，在整个72小时的增长，在体内的敏感性DNase I消化和转录水平。DNA酶I敏感性与转录水平呈正相关，这意味着它是基因表达的预测，并表明转录DNA的可及性增加。基于对DNA酶I消化的敏感性将基因组分级，与代表整个基因组的不频繁切割的级分相比，低分子量（频繁切割）级分高度富集活性转录序列。这种方法将允许比较与细菌基因组的转录活性和非活性区域相关的类核蛋白及其任何修饰。
For a bacterium, Streptomyces coelicolor A3(2) contains a relatively large genome (8.7 Mb) with a complex and adaptive pattern of gene regulation. We discovered a correlation between the physical structure of the S.coelicolor genome and the transcriptional activity of the genes therein. Twelve genes were surveyed throughout 72 h of growth for both in vivo sensitivity to DNase I digestion and levels of transcription. DNase I-sensitivity correlated positively with transcript levels, implying that it was predictive of gene expression, and indicating increased accessibility of transcribed DNA. The genome was fractionated based on the sensitivity to DNase I digestion, with the low molecular weight (frequently cut) fraction highly enriched for actively transcribed sequences when compared to the infrequently cut fraction, which was representative of the entire genome. This approach will allow comparison of nucleoid proteins, and any modifications thereof, associated with transcriptionally active and inactive regions of the bacterial genome.
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影响因子：
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作者：
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