The Mechanism Forming the Cell Surface of Tip-Growing Rooting Cells Is Conserved among Land Plants.
The Mechanism Forming the Cell Surface of Tip-Growing Rooting Cells Is Conserved among Land Plants.
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DOI：
10.1016/j.cub.2016.09.062
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发表时间：
2016-12-05
期刊：
影响因子：
9.2
通讯作者：
Dolan, Liam
中科院分区：
文献类型：
--
作者：
Honkanen, Suvi;Jones, Victor A. S.;Morieri, Giulia;Champion, Clement;Hetherington, Alexander J.;Kelly, Steve;Proust, Helene;Saint-Marcoux, Denis;Prescott, Helen;Dolan, Liam
为了发现控制最早陆地植物生根系统生长的机制，我们在多形地茅（Marchantia polymorpha）中鉴定了控制类根发育的基因。对336,000个T-DNA转化系进行了根茎生长缺陷突变体筛选，并生成了一个从头基因组组装来鉴定突变基因。我们报告了33个根状生长所需的基因的鉴定，其中6个以前没有在绿色植物中功能表征。研究表明，在多形根和拟南芥根毛生长过程中，同一正群的成员在细胞壁合成、细胞壁完整性感知和囊泡运输中都很活跃。这表明顶端生长的生根细胞的细胞表面构造机制在陆生植物中是保守的，并且在4.7亿年前最早的陆生植物中是活跃的。336000本文分别行和基因组组装生成Marchantia polymorpha 33假根增长所需的基因被确定六33基因的功能特点首次在植物基因属于这些orthogroups活跃在第一个土地植物根系Honkanen等人确定33基因假根支持细胞的生长所需的苔类Marchantia polymorpha 336000年屏幕T-DNA-mutagenized线条和使用新创基因组组装。相关基因在4.6亿年前第一个植物生根结构的发育过程中是活跃的。
To discover mechanisms that controlled the growth of the rooting system in the earliest land plants, we identified genes that control the development of rhizoids in the liverwort Marchantia polymorpha. 336,000 T-DNA transformed lines were screened for mutants with defects in rhizoid growth, and a de novo genome assembly was generated to identify the mutant genes. We report the identification of 33 genes required for rhizoid growth, of which 6 had not previously been functionally characterized in green plants. We demonstrate that members of the same orthogroup are active in cell wall synthesis, cell wall integrity sensing, and vesicle trafficking during M. polymorpha rhizoid and Arabidopsis thaliana root hair growth. This indicates that the mechanism for constructing the cell surface of tip-growing rooting cells is conserved among land plants and was active in the earliest land plants that existed sometime more than 470 million years ago. 336,000 T-DNA lines and a genome assembly were generated in Marchantia polymorpha 33 genes required for rhizoid growth were identified Six of the 33 genes were functionally characterized in plants for the first time Genes belonging to these orthogroups were active in the first land plant roots Honkanen et al. identify 33 genes required for the growth of rhizoid rooting cells in the liverwort Marchantia polymorpha in a screen of 336,000 T-DNA-mutagenized lines and using a de novo genome assembly. Related genes were active during the development of the first plant rooting structures sometime before 460 million years ago.
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影响因子：
11.6
作者：
Diet, Anouck;Link, Bruce;Ringli, Christoph
通讯作者：
Ringli, Christoph
DOI：
10.1073/pnas.1005366107
发表时间：
2010-10-12
影响因子：
11.1
作者：
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影响因子：
11.6
作者：
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通讯作者：
Klein, Markus
影响因子：
7.2
作者：
Saedler, Rainer;Jakoby, Marc;Huelskamp, Martin
通讯作者：
Huelskamp, Martin