Systematic mapping of contact sites reveals tethers and a function for the peroxisome-mitochondria contact.
Systematic mapping of contact sites reveals tethers and a function for the peroxisome-mitochondria contact.
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DOI：
10.1038/s41467-018-03957-8
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发表时间：
2018-05-02
影响因子：
16.6
通讯作者：
Zalckvar E
中科院分区：
文献类型：
--
作者：
Shai N;Yifrach E;van Roermund CWT;Cohen N;Bibi C;IJlst L;Cavellini L;Meurisse J;Schuster R;Zada L;Mari MC;Reggiori FM;Hughes AL;Escobar-Henriques M;Cohen MM;Waterham HR;Wanders RJA;Schuldiner M;Zalckvar E
The understanding that organelles are not floating in the cytosol, but rather held in an organized yet dynamic interplay through membrane contact sites, is altering the way we grasp cell biological phenomena. However, we still have not identified the entire repertoire of contact sites, their tethering molecules and functions. To systematically characterize contact sites and their tethering molecules here we employ a proximity detection method based on split fluorophores and discover four potential new yeast contact sites. We then focus on a little-studied yet highly disease-relevant contact, the Peroxisome-Mitochondria (PerMit) proximity, and uncover and characterize two tether proteins: Fzo1 and Pex34. We genetically expand the PerMit contact site and demonstrate a physiological function in β-oxidation of fatty acids. Our work showcases how systematic analysis of contact site machinery and functions can deepen our understanding of these structures in health and disease. The internal organization of the cell has been enriched by the discovery that organelles establish membrane contact sites, however the entire repertoire of these contacts is still being explored. Here the authors systematically identify the landscape of cellular contact sites in yeast, discovering four potential novel contact sites and two tether proteins for the peroxisome-mitochondria contact site.
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影响因子：
--
作者：
Cohen, Yifat;Klug, Yoel Alexander;Schuldiner, Maya
通讯作者：
Schuldiner, Maya
影响因子：
8.8
作者：
Elbaz-Alon Y;Eisenberg-Bord M;Shinder V;Stiller SB;Shimoni E;Wiedemann N;Geiger T;Schuldiner M
通讯作者：
Schuldiner M
影响因子：
9.2
作者：
Daniele, Tiziana;Hurbain, Ilse;Schiaffino, Maria Vittoria
通讯作者：
Schiaffino, Maria Vittoria
DOI：
10.1007/978-1-61779-276-2_8
发表时间：
2011-01-01
期刊：
NETWORK BIOLOGY: METHODS AND APPLICATIONS
影响因子：
--
作者：
Cohen, Yifat;Schuldiner, Maya
通讯作者：
Schuldiner, Maya
影响因子：
4
作者：
Anton, Fabian;Fres, Julia M.;Escobar-Henriques, Mafalda
通讯作者：
Escobar-Henriques, Mafalda