Preparation of antibacterial and osteoconductive 3D-printed PLGA/Cu(I)@ZIF-8 nanocomposite scaffolds for infected bone repair
Preparation of antibacterial and osteoconductive 3D-printed PLGA/Cu(I)@ZIF-8 nanocomposite scaffolds for infected bone repair
复制标题
用于感染骨修复的抗菌和骨传导性3D打印PLGA/Cu(I)@ZIF-8纳米复合材料支架的制备
DOI：
10.1186/s12951-020-00594-6
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发表时间：
2020-02
影响因子：
10.2
通讯作者：
Xiaosheng Ma
中科院分区：
文献类型：
--
作者：
Fei Zou;Jianyuan Jiang;Feizhou Lv;Xinlei Xia;Xiaosheng Ma
背景
大面积骨缺损的修复是临床上的一大挑战。在这项研究中，载铜ZIF-8纳米颗粒和聚（丙交酯-共-乙交酯）（PLGA）相结合，利用三维打印技术制备多孔PLGA/Cu（I）@ZIF-8支架，用于感染骨修复。
方法
通过透射电镜和扫描电镜观察PLGA/Cu（I）@ZIF-8支架的表面形貌。将PLGA/Cu（I）@ZIF-8支架与细菌共培养以确定其抗菌性能，并与小鼠间充质干细胞（MSC）共培养以探索其生物相容性和骨传导性能。通过在模拟体液中孵育来评价PLGA/Cu（I）@ZIF-8支架的生物活性。
结果
结果表明，PLGA/Cu（I）@ZIF-8支架的孔隙率为80.04 ± 5.6%，具有良好的力学性能。当与H2 O2孵育时，Cu（I）@ZIF-8纳米颗粒产生活性氧物质，这有助于其抗菌性能。与单纯PLGA支架相比，PLGA/Cu（I）@ZIF-8支架表面培养的mMSCs贴壁、铺展良好，增殖率高，碱性磷酸酶和茜素红染色增强。矿化实验显示PLGA/Cu（I）@ZIF-8支架表面形成了一层富含磷灰石的层，而PLGA支架表面几乎没有磷灰石。此外，在体外，在PLGA/Cu（I）@ZIF-8支架上培养的金黄色葡萄球菌几乎全部死亡，而在植入PLGA/Cu@ZIF-8支架的感染大鼠中，体内炎性细胞浸润和细菌数量显著减少。
结论
这些结果表明PLGA/Cu（I）@ZIF-8支架具有优异的抗菌和骨传导性能，以及良好的生物相容性和高生物活性。本研究表明PLGA/Cu（I）@ZIF-8支架材料可作为一种有前途的骨组织工程生物材料，特别是用于感染骨的修复。
Background
The repair of large bone defects is a great challenge in clinical practice. In this study, copper-loaded-ZIF-8 nanoparticles and poly (lactide-co-glycolide) (PLGA) were combined to fabricate porous PLGA/Cu(I)@ZIF-8 scaffolds using three-dimensional printing technology for infected bone repair.
Methods
The surface morphology of PLGA/Cu(I)@ZIF-8 scaffolds was investigated by transmission electron microscopy and scanning electron microscopy. The PLGA/Cu(I)@ZIF-8 scaffolds were co-cultured with bacteria to determine their antibacterial properties, and with murine mesenchymal stem cells (MSCs) to explore their biocompatibility and osteoconductive properties. The bioactivity of the PLGA/Cu(I)@ZIF-8 scaffolds was evaluated by incubating in simulated body fluid.
Results
The results revealed that the PLGA/Cu(I)@ZIF-8 scaffolds had porosities of 80.04 ± 5.6% and exhibited good mechanical properties. When incubated with H2O2, Cu(I)@ZIF-8 nanoparticles resulted generated reactive oxygen species, which contributed to their antibacterial properties. The mMSCs cultured on the surface of PLGA/Cu(I)@ZIF-8 scaffolds were well-spread and adherent with a high proliferation rate, and staining with alkaline phosphatase and alizarin red was increased compared with the pure PLGA scaffolds. The mineralization assay showed an apatite-rich layer was formed on the surface of PLGA/Cu(I)@ZIF-8 scaffolds, while there was hardly any apatite on the surface of the PLGA scaffolds. Additionally, in vitro, Staphylococcus aureus cultured on the PLGA/Cu(I)@ZIF-8 scaffolds were almost all dead, while in vivo inflammatory cell infiltration and bacteria numbers were dramatically reduced in infected rats implanted with PLGA/Cu@ZIF-8 scaffolds.
Conclusion
All these findings demonstrate that PLGA/Cu(I)@ZIF-8 scaffolds possess excellent antibacterial and osteoconductive properties, as well as good biocompatibility and high bioactivity. This study suggests that the PLGA/Cu(I)@ZIF-8 scaffolds could be used as a promising biomaterial for bone tissue engineering, especially for infected bone repair.
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