Leishmania major-Infected Phlebotomus duboscqi Sand Fly Bites Enhance Mast Cell Degranulation.
Leishmania major-Infected Phlebotomus duboscqi Sand Fly Bites Enhance Mast Cell Degranulation.
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DOI：
10.3390/pathogens12020207
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发表时间：
2023-01-28
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影响因子：
--
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中科院分区：
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利什曼原虫通过白蛉媒介的叮咬感染哺乳动物宿主。肥大细胞（MC）的寄生虫和媒介衍生的因子，白蛉叮咬提供的反应，尚未得到表征。我们分析了在BALB/c小鼠自然感染利什曼原虫的耳朵，通过白蛉媒介Phlebotomus duboscqi的叮咬MC的数量和他们的介质，并将它们与未感染的白蛉叮咬进行比较。在感染和未感染的白蛉的叮咬部位发现MC在整个48 h内，显示具有强烈的TNF-α、组胺和类胰蛋白酶染色的颗粒的释放。在30分钟和48小时，与未感染叮咬或对照相比，感染的MC数量显著更高（p < 0.001）。在感染和未感染的沙蝇叮咬的皮肤中，神经元的募集在前6小时内是强烈的，此后减少。淋巴结中也出现中性粒细胞流入，在单核细胞中观察到强烈的TNF-α染色。我们的数据表明，MC精心策划感染和未感染的白蛉叮咬后的早期炎症反应，导致嗜中性招募，这可能为哺乳动物宿主内的寄生虫提供安全通道。
Leishmania parasites infect mammalian hosts through the bites of sand fly vectors. The response by mast cells (MC) to the parasite and vector-derived factors, delivered by sand fly bites, has not been characterized. We analyzed MC numbers and their mediators in BALB/c mice naturally infected in the ear with Leishmania major through the bite of the sand fly vector Phlebotomus duboscqi and compared them to non-infected sand fly bites. MC were found at the bite sites of infective and non-infected sand flies throughout 48 h, showing the release of granules with intense TNF-α, histamine, and tryptase staining. At 30 min and 48 h, the MC numbers were significantly higher (p < 0.001) in infected as compared to non-infected bites or controls. Neutrophil recruitment was intense during the first 6 h in the skin of infected and non-infected sand fly bites and decreased thereafter. An influx of neutrophils also occurred in lymph nodes, where a strong TNF-α stain was observed in mononuclear cells. Our data show that MC orchestrate an early inflammatory response after infected and non-infected sand fly bites, leading to neutrophilic recruitment, which potentially provides a safe passage for the parasite within the mammalian host.
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感染期间淋巴结中中性粒细胞迁移的动态。
影响因子：
32.4
作者：
Chtanova, Tatyana;Schaeffer, Marie;Han, Seong-Ji;van Dooren, Giel G.;Nollmann, Marcelo;Herzmark, Paul;Chan, Shiao Wei;Satija, Harshita;Camfield, Kristin;Aaron, Holly;Striepen, Boris;Robey, Ellen A.
通讯作者：
Robey, Ellen A.
影响因子：
2.8
作者：
DaCruz, AM;deOliveira, MP;Coutinho, SG
通讯作者：
Coutinho, SG
DOI：
10.1038/nri2782
发表时间：
2010-06
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
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影响因子：
15.3
作者：
Beghdadi, Walid;Porcherie, Adeline;Schneider, Bradley S.;Dubayle, David;Peronet, Roger;Huerre, Michel;Watanabe, Takeshi;Ohtsu, Hiroshi;Louis, Jacques;Mecheri, Salaheddine
通讯作者：
Mecheri, Salaheddine
影响因子：
16.6
作者：
Guimaraes-Costa AB;Shannon JP;Waclawiak I;Oliveira J;Meneses C;de Castro W;Wen X;Brzostowski J;Serafim TD;Andersen JF;Hickman HD;Kamhawi S;Valenzuela JG;Oliveira F
通讯作者：
Oliveira F