Peripheral blood in Sjögren's syndrome does not contain increased levels of T lymphocytes reactive with the recombinant Ro/SS-A 52 kD and La/SS-B 48 kD autoantigens.
Peripheral blood in Sjögren's syndrome does not contain increased levels of T lymphocytes reactive with the recombinant Ro/SS-A 52 kD and La/SS-B 48 kD autoantigens.
复制标题
干燥综合征的外周血中与重组 Ro/SS-A 52 kD 和 La/SS-B 48 kD 自身抗原反应的 T 淋巴细胞水平不升高。
作者：
A. Halse;M. Wahren;R. Jonsson
干燥综合征 (SS) 患者经常具有抗 Ro/SS-A 和抗 La/SS-B 自身抗体。本研究的目的是调查这些患者在 Ro/SS-A 和 La/SS-B 抗原存在下短期培养后是否具有分泌 IFN-γ 的外周血淋巴细胞 (PBL)。在 12 名 SS 患者和 11 名健康对照中检查了 PBL 分泌 IFN-γ 的频率。在重组 Ro 52 kD 蛋白或重组 La 48 kD 蛋白存在下，PBL 培养 48 小时后进行酶联免疫斑点 (ELISPOT) 测定。 SS患者组中未刺激的IFN-γ分泌细胞的数量与对照组没有显着差异。此外，在Ro/SS-A和La/SS-B刺激后，两组中均未检测到IFN-γ分泌细胞数量增加。因此，在 SS 患者的外周血中，T 细胞与重组 Ro 52 kD 和 La 48 kD 蛋白发生反应的频率不会增加。
Patients with Sjögren's syndrome (SS) frequently have anti-Ro/SS-A and anti-La/SS-B autoantibodies. The aim of this study was to investigate if these patients have peripheral blood lymphocytes (PBL) secreting IFN-gamma after short-term cultivation in the presence of Ro/SS-A and La/SS-B antigens. The frequency of PBL secreting IFN-gamma was examined in 12 SS patients and 11 healthy controls. The enzyme-linked immunospot (ELISPOT) assay was performed after 48 hours cultivation of PBL in the presence of recombinant Ro 52 kD protein or recombinant La 48 kD protein. The number of unstimulated IFN-gamma secreting cells in the SS patient group was not significantly different from that of the control group. Moreover, no increase in the number of IFN-gamma secreting cells after Ro/SS-A and La/SS-B stimulation was detected in the two groups. Thus, T cells reactive with the recombinant Ro 52 kD and La 48 kD proteins do not occur with any increased frequency in peripheral blood of SS patients.
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影响因子：
--
作者：
BENCHETRIT, E;FOX, RI;TAN, EM
通讯作者：
TAN, EM
Ro(SSA) 颗粒的分子定义：系统性红斑狼疮和干燥综合征中自身免疫的常见目标。
DOI：
--
发表时间：
1991
期刊：
British journal of rheumatology
影响因子：
--
作者：
Reichlin,M
通讯作者：
Reichlin,M
DOI：
10.1073/pnas.81.7.1996
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
WOLIN, SL;STEITZ, JA
通讯作者：
STEITZ, JA
与干燥综合征相关的原发性胆汁性肝硬化：循环和组织沉积 Ro/抗 Ro 免疫复合物的证据。
影响因子：
--
作者：
Penner,E;Reichlin,M
通讯作者：
Reichlin,M