Phase-specific plasticity of synaptic structures in the somatosensory cortex of living mice during neuropathic pain.
Phase-specific plasticity of synaptic structures in the somatosensory cortex of living mice during neuropathic pain.
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DOI：
10.1186/1744-8069-7-87
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发表时间：
2011-11-09
期刊：
影响因子：
3.3
通讯作者：
Nabekura J
中科院分区：
文献类型：
--
作者：
Kim SK;Kato G;Ishikawa T;Nabekura J
大脑皮质中的突触后树突棘是高度动态的，随着感觉输入或神经元活动的改变，表现出快速的形态变化，包括延长/回缩和形成/消除，从而实现经验/活动依赖的皮质回路重新布线。我们先前的体内长期双光子成像研究显示，在神经病理性疼痛的早期发展阶段，小鼠初级躯体感觉(S1)皮质的脊柱翻转显著增加，但在神经病理性疼痛的后期维持阶段恢复。然而，尚不清楚在翻转改变之前脊柱形态是如何改变的，以及突触前突触的获得和丢失是否在神经病理性疼痛期间发生改变。在这里，我们使用了体内短期(2小时)和长期(2周)时间推移的双光子成像，对部分坐骨神经结扎(PSL)后在锥体神经元中表达GFP的转基因小鼠的S1皮层第一层的单个脊椎和脊椎进行了双光子成像。我们在短期成像中发现，在神经病理性疼痛的发展阶段，脊柱运动(每30分钟的Δ长度)显著增加，但在维持阶段恢复到基线水平。此外，未成熟(细)和成熟(蘑菇)刺的比例仅在发育阶段分别增加和减少。长期的成像数据显示，在PSL后的前3天，结节的形成和消除分别适度增加和减少，随后恢复。我们的结果表明，在PSL后，S1突触结构迅速失稳和重新排列，随后在神经病理性疼痛的维持期稳定下来，这为顽固性慢性疼痛提供了一个新的治疗靶点。
Postsynaptic dendritic spines in the cortex are highly dynamic, showing rapid morphological changes including elongation/retraction and formation/elimination in response to altered sensory input or neuronal activity, which achieves experience/activity-dependent cortical circuit rewiring. Our previous long-term in vivo two-photon imaging study revealed that spine turnover in the mouse primary somatosensory (S1) cortex markedly increased in an early development phase of neuropathic pain, but was restored in a late maintenance phase of neuropathic pain. However, it remains unknown how spine morphology is altered preceding turnover change and whether gain and loss of presynaptic boutons are changed during neuropathic pain. Here we used short-term (2-hour) and long-term (2-week) time-lapse in vivo two-photon imaging of individual spines and boutons in the S1 cortical layer 1 of the transgenic mice expressing GFP in pyramidal neurons following partial sciatic nerve ligation (PSL). We found in the short-term imaging that spine motility (Δ length per 30 min) significantly increased in the development phase of neuropathic pain, but returned to the baseline in the maintenance phase. Moreover, the proportion of immature (thin) and mature (mushroom) spines increased and decreased, respectively, only in the development phase. Long-term imaging data showed that formation and elimination of boutons moderately increased and decreased, respectively, during the first 3 days following PSL and was subsequently restored. Our results indicate that the S1 synaptic structures are rapidly destabilized and rearranged following PSL and subsequently stabilized in the maintenance phase of neuropathic pain, suggesting a novel therapeutic target in intractable chronic pain.
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DOI：
10.1523/jneurosci.1661-10.2010
发表时间：
2010-08-18
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Tropea D;Majewska AK;Garcia R;Sur M
通讯作者：
Sur M
影响因子：
14.8
作者：
Holtmaat, Anthony;Bonhoeffer, Tobias;Chow, David K.;Chuckowree, Jyoti;De Paola, Vincenzo;Hofer, Sonja B.;Huebener, Mark;Keck, Tara;Knott, Graham;Lee, Wei-Chung A.;Mostany, Ricardo;Mrsic-Flogel, Tom D.;Nedivi, Elly;Portera-Cailliau, Carlos;Svoboda, Karel;Trachtenberg, Joshua T.;Wilbrecht, Linda
通讯作者：
Wilbrecht, Linda
影响因子：
64.8
作者：
Engert, F;Bonhoeffer, T
通讯作者：
Bonhoeffer, T
影响因子：
5.3
作者：
Majewska, AK;Newton, JR;Sur, M
通讯作者：
Sur, M
影响因子：
13.9
作者：
Costigan M;Scholz J;Woolf CJ
通讯作者：
Woolf CJ