Human Subacromial Bursal Cells Display Superior Engraftment Versus Bone Marrow Stromal Cells in Murine Tendon Repair.
Human Subacromial Bursal Cells Display Superior Engraftment Versus Bone Marrow Stromal Cells in Murine Tendon Repair.
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DOI：
10.1177/0363546518802842
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Dyment NA
中科院分区：
文献类型：
--
作者：
Dyrna F;Zakko P;Pauzenberger L;McCarthy MB;Mazzocca AD;Dyment NA
骨髓抽吸物是细胞疗法的主要来源，在骨科手术领域具有越来越大的价值，特别是在肌腱和韧带修复的翻修病例中。然而，腱周结构内的细胞，例如腱旁和周围的滑囊，有助于天然肌腱愈合反应，并为基于细胞的修复策略提供有前途的细胞群。因此，本研究的目的是在已建立的体内免疫缺陷小鼠髌腱缺损模型中，研究与当前金标准骨髓基质细胞（BMSC）相比，源自人肩峰下囊的细胞对于肌腱修复的功效。与骨髓间充质干细胞相比，肩峰下法氏囊细胞将表现出优异的存活率和植入宿主组织的能力。受控实验室研究。人类肩峰下法氏囊和骨髓抽吸物是从进行肩袖修复的同一供体中采集的。用荧光慢病毒载体转染细胞，永久标记细胞，封装到纤维蛋白凝胶中，植入免疫缺陷小鼠双侧全长中央宽度髌腱缺损处。对对照小鼠进行额外的手术，比较没有细胞的纤维蛋白和自然愈合。处死时，用多光子显微镜扫描所有四肢，以监测人类供体细胞的植入情况。之后，四肢被分配进行免疫组织化学或生物力学分析。与 BMSC 相比，植入的肩峰下法氏囊细胞表现出优异的组织移植和存活率。该缺损模型中的主要愈合反应是在缺损空间的前表面上产生新的愈合组织。与未接受细胞的对照肢体相比，细胞植入显着增加了前部愈合组织的厚度。接受植入细胞的肢体中的细胞增殖也有所增加，这表明供体细胞刺激了更强烈的愈合反应。最后，愈合反应的这些变化并没有导致机械性能的显着变化。肩峰下滑囊虽然在肩袖修复过程中经常被移除，但可能比骨髓间充质干细胞更适合肌腱修复，因为滑囊细胞在肌腱组织中表现出优异的植入和存活能力。此外，肩峰下囊可能是比骨髓抽吸更容易获得的细胞来源。肩峰下囊含有对肌腱损伤做出反应的细胞群，可能为肌腱修复和再生策略提供更佳的细胞来源。因此，未来可以从该组织中收获细胞，而不是目前的滑囊切除术和清创术。
Bone marrow aspirate is a primary source for cell-based therapies with increasing value in the world of orthopaedic surgery, especially in revision cases of tendon and ligament repairs. However, cells within peritendinous structures, such as the paratenon and surrounding bursa, contribute to the native tendon-healing response and offer promising cell populations for cell-based repair strategies. Therefore, the purpose of this study is to investigate the efficacy of cells derived from human subacromial bursa as compared with the current gold standard, bone marrow stromal cells (BMSCs), for tendon repairs in an established in vivo immunodeficient murine patellar tendon defect model. Subacromial bursal cells will show superior survival and engraftment into the host tissue as compared with BMSCs. Controlled laboratory study. Human subacromial bursal and bone marrow aspirate were harvested from the same donor undergoing rotator cuff repair. Cells were transfected with a fluorescent lentiviral vector to permanently label the cells, encapsulated into fibrin gel, and implanted into bilateral full-length central-width patellar tendon defects of immunodeficient mice. Additional surgery was performed on control mice comparing fibrin without cells and natural healing. At the time of sacrifice, all limbs were scanned on a multiphoton microscope to monitor the engraftment of the human donor cells. Afterward, limbs were assigned to either immunohistochemical or biomechanical analysis. As compared with BMSCs, implanted subacromial bursal cells displayed superior tissue engraftment and survival. The main healing response in this defect model was the creation of new healing tissue over the anterior surface of the defect space. The implantation of cells significantly increased the thickness of the anterior healing tissue as compared with control limbs that did not receive cells. Cell proliferation was also increased in limbs that received implanted cells, suggesting that the donor cells stimulated a more robust healing response. Finally, these changes in the healing response did not lead to significant changes in mechanical properties. The subacromial bursa, while often removed during rotator cuff repair, may harbor a more suitable cell source for tendon repair than BMSCs, as bursal cells display superior engraftment and survival in tendon tissue. In addition, the subacromial bursa may be a more accessible cell source than bone marrow aspirate. The subacromial bursa contains a cell population that responds to tendon injury and may provide a more optimal cell source for tendon repair and regeneration strategies. Therefore, cells could be harvested from this tissue in the future, as opposed to the current practice of bursectomy and debridement.
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影响因子：
3.8
作者：
Ellera Gomes, Joao L.;da Silva, Ricardo Canquerini;Silla, Lucia M. R.;Abreu, Marcelo R.;Pellanda, Roberto
通讯作者：
Pellanda, Roberto
影响因子：
4.8
作者：
Mazzocca, Augustus D.;McCarthy, Mary Beth R.;Drissi, Hichann
通讯作者：
Drissi, Hichann
影响因子：
4.8
作者：
Huang, Tung-Fu;Yew, Tu-Lai;Hung, Shih-Chieh
通讯作者：
Hung, Shih-Chieh
影响因子：
82.9
作者：
Bi, Yanming;Ehirchiou, Driss;Young, Marian F.
通讯作者：
Young, Marian F.
DOI：
10.1002/jor.23064
发表时间：
2016-04
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Gelberman RH;Shen H;Kormpakis I;Rothrauff B;Yang G;Tuan RS;Xia Y;Sakiyama-Elbert S;Silva MJ;Thomopoulos S
通讯作者：
Thomopoulos S