Chronic exposure to ambient particulate matter induces gut microbial dysbiosis in a rat COPD model.
Chronic exposure to ambient particulate matter induces gut microbial dysbiosis in a rat COPD model.
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慢性暴露于环境颗粒物会导致大鼠慢性阻塞性肺病模型肠道微生物失调
DOI：
10.1186/s12931-020-01529-3
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发表时间：
2020-10-19
影响因子：
5.8
通讯作者：
Ran P
中科院分区：
文献类型：
--
作者：
Li N;Yang Z;Liao B;Pan T;Pu J;Hao B;Fu Z;Cao W;Zhou Y;He F;Li B;Ran P
BackgroundThe作用的微生物在慢性阻塞性肺疾病（COPD）的发病机制暴露于环境颗粒物（PM）在很大程度上是unknown. MethodsFifty 4雄性Sprague-Dawley大鼠暴露于清洁空气，生物质燃料（BMF），或机动车尾气（MVE）为4，12和24周。我们在暴露期间的三个不同时间点对大鼠肺进行了肺部炎症评价、形态测量和肺功能分析。通过16 S rRNA焦磷酸测序评估肺和肠道微生物组成。血清脂多糖水平进行了测定，结肠内容物中的短链脂肪酸进行了quantited.ResultsAfter 24周PM曝光，大鼠表现出COPD的肺部炎症和病理变化的特点。对照组和PM暴露组（BMF和MVE）大鼠肺中的微生物多样性和组成相似。然而，24周PM暴露后的肠道菌群的特点是减少微生物的丰富性和多样性，不同的整体微生物组成，短链脂肪酸的水平较低，血清lipopolysaccharide.Conclusionchronic暴露于环境颗粒物诱导肠道微生物生态失调和代谢物的变化在大鼠模型的慢性阻塞性肺疾病。
BackgroundThe role of the microbiota in the pathogenesis of chronic obstructive pulmonary disease (COPD) following exposure to ambient particulate matter (PM) is largely unknown.MethodsFifty-four male Sprague-Dawley rats were exposed to clean air, biomass fuel (BMF), or motor vehicle exhaust (MVE) for 4, 12, and 24 weeks. We performed pulmonary inflammation evaluation, morphometric measurements, and lung function analysis in rat lung at three different times points during exposure. Lung and gut microbial composition was assessed by 16S rRNA pyrosequencing. Serum lipopolysaccharide levels were measured and short-chain fatty acids in colon contents were quantified.ResultsAfter a 24-week PM exposure, rats exhibited pulmonary inflammation and pathological changes characteristic of COPD. The control and PM exposure (BMF and MVE) groups showed similar microbial diversity and composition in rat lung. However, the gut microbiota after 24 weeks PM exposure was characterized by decreased microbial richness and diversity, distinct overall microbial composition, lower levels of short-chain fatty acids, and higher serum lipopolysaccharide.ConclusionChronic exposure to ambient particulate matter induces gut microbial dysbiosis and metabolite shifts in a rat model of chronic obstructive pulmonary disease.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
暴露于环境颗粒物会改变微生物组成并诱导大鼠肺部的免疫变化。
影响因子：
5.8
作者：
Li N;He F;Liao B;Zhou Y;Li B;Ran P
通讯作者：
Ran P
DOI：
10.1111/j.1475-097x.2011.01058.x
发表时间：
2012-01-01
影响因子：
1.8
作者：
Korsgren, Magnus;Linden, Margareta;Greiff, Lennart
通讯作者：
Greiff, Lennart
影响因子：
9.8
作者：
Li, Jingli;Hu, Yiran;Chen, Chengshui
通讯作者：
Chen, Chengshui
影响因子：
5.8
作者：
Bouquet, Jerome;Tabor, David E.;Jin, Hong
通讯作者：
Jin, Hong