Exposure to ambient particulate matter alters the microbial composition and induces immune changes in rat lung.
Exposure to ambient particulate matter alters the microbial composition and induces immune changes in rat lung.
复制标题
暴露于环境颗粒物会改变微生物组成并诱导大鼠肺部的免疫变化。
DOI：
10.1186/s12931-017-0626-6
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发表时间：
2017-07-25
影响因子：
5.8
通讯作者：
Ran P
中科院分区：
文献类型：
--
作者：
Li N;He F;Liao B;Zhou Y;Li B;Ran P
环境颗粒物暴露已被证明会增加呼吸系统疾病的风险。然而，肺部微生物组的作用和对吸入颗粒物的免疫反应在很大程度上尚未探索。我们研究了生物质燃料和机动车尾气颗粒物对大鼠肺微生物组和肺免疫稳态的影响。将57只SD大鼠随机分为清洁空气组（CON）、生物质燃料组（BMF）和机动车尾气组（MVE）。暴露4周后，通过16S rRNA焦磷酸测序评估肺的微生物组成，通过组织学分析评估肺组织的结构，通过流式细胞术确定肺泡巨噬细胞对细菌的吞噬反应，并使用商业ELISA试剂盒测量免疫球蛋白浓度。两组之间的肺形态无显著差异。然而，BMF和MVE组显示出更大的细菌丰度和多样性。变形菌在MVE组中的比例较高，12个细菌家族在三组之间的相对丰度不同。此外，颗粒物暴露显著增加肺泡巨噬细胞吞噬细菌的能力，并诱导免疫球蛋白水平的变化。我们证明，颗粒物暴露可以改变微生物的组成和改变肺免疫稳态大鼠肺。
Ambient particulate matter exposure has been shown to increase the risks of respiratory diseases. However, the role of the lung microbiome and the immune response to inhaled particulate matter are largely unexplored. We studied the influence of biomass fuel and motor vehicle exhaust particles on the lung microbiome and pulmonary immunologic homeostasis in rats. Fifty-seven Sprague–Dawley rats were randomly divided into clean air (CON), biomass fuel (BMF), and motor vehicle exhaust (MVE) groups. After a 4-week exposure, the microbial composition of the lung was assessed by 16S rRNA pyrosequencing, the structure of the lung tissue was assessed with histological analysis, the phagocytic response of alveolar macrophages to bacteria was determined by flow cytometry, and immunoglobulin concentrations were measured with commercial ELISA kits. There was no significant difference in lung morphology between the groups. However, the BMF and MVE groups displayed greater bacterial abundance and diversity. Proteobacteria were present in higher proportions in the MVE group, and 12 bacterial families differed in their relative abundances between the three groups. In addition, particulate matter exposure significantly increased the capacity of alveolar macrophages to phagocytose bacteria and induced changes in immunoglobulin levels. We demonstrated that particulate matter exposure can alter the microbial composition and change the pulmonary immunologic homeostasis in the rat lung.
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DOI：
10.1165/rcmb.2015-0017oc
发表时间：
2016-03-01
影响因子：
6.4
作者：
Basilico, Paola;Cremona, Tiziana P.;Benarafa, Charaf
通讯作者：
Benarafa, Charaf
影响因子：
3.7
作者：
Erb-Downward JR;Thompson DL;Han MK;Freeman CM;McCloskey L;Schmidt LA;Young VB;Toews GB;Curtis JL;Sundaram B;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
影响因子：
15.5
作者：
Fadrosh DW;Ma B;Gajer P;Sengamalay N;Ott S;Brotman RM;Ravel J
通讯作者：
Ravel J
DOI：
10.1098/rstb.1994.0085
发表时间：
1994-07-29
影响因子：
6.3
作者：
FAITH, DP
通讯作者：
FAITH, DP
影响因子：
11.4
作者：
Jaligama S;Chen Z;Saravia J;Yadav N;Lomnicki SM;Dugas TR;Cormier SA
通讯作者：
Cormier SA