Molecular architecture of tight junctions of periderm differs from that of the maculae occludentes of epidermis.
Molecular architecture of tight junctions of periderm differs from that of the maculae occludentes of epidermis.
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周皮紧密连接的分子结构与表皮闭塞斑的分子结构不同。
DOI：
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发表时间：
2002
影响因子：
6.5
通讯作者：
Y. Miyachi
中科院分区：
文献类型：
--
作者：
K. Morita;M. Furuse;Y. Yoshida;M. Itoh;H. Sasaki;S. Tsukita;Y. Miyachi
Occludin和claudins是紧密连接中的四环素跨膜蛋白。闭塞斑是一种不太发达的紧密连接，发生在表皮的颗粒细胞层。周皮覆盖在发育中的表皮之上，作为胚胎的保护层，与简单上皮中观察到的那样，带有发达的紧密连接。在周皮和表皮中，occludin在细胞-细胞交界处表达。为了确定周皮和表皮紧密连接的区别，我们检测了claudin-6在周皮和表皮中的表达。免疫荧光染色显示Claudin-6在周皮的细胞-细胞交界处有表达，而在表皮未见表达。逆转录聚合酶链式反应证实Claudin-6在成年小鼠皮肤中不表达，而免疫电子显微镜显示Claudin-6定位于周皮的紧密连接。此外，稳定表达外源性claudin-6的L成纤维细胞在细胞-细胞边界形成紧密连接。这些发现表明，周皮紧密连接的分子结构不同于表皮闭塞斑点的分子结构，Claudin-6在周皮紧密连接的形成中起重要作用。
Occludin and claudins are tetraspan-transmembrane proteins in tight junctions. Maculae occludentes, which are less-developed tight junctions, occur in the granular cell layer of the epidermis. The periderm, which overlies the developing epidermis and functions as a protective layer for the embryo, carries developed tight junctions as observed in simple epithelia. In both periderm and epidermis, occludin is expressed at the cell-cell border. To determine the difference between tight junctions of periderm and epidermis, claudin-6 expression was examined in periderm and epidermis. Immunofluorescence staining showed claudin-6 expression at the cell-cell border of the periderm, but not in the epidermis. Reverse transcription-polymerase chain reaction confirmed that claudin-6 was not expressed in mouse adult skin, whereas immunoelectron microscopy revealed that claudin-6 was localized at tight junctions of the periderm. Furthermore, L fibroblasts with stable expression of exogenous claudin-6 formed developed tight junctions at cell-cell borders. These findings indicate that molecular architecture of tight junctions of the periderm is different from that of the maculae occludentes of the epidermis, and that claudin-6 is important in the formation of tight junctions of the periderm.
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影响因子：
56.9
作者：
RODRIGUEZBOULAN, E;NELSON, WJ
通讯作者：
NELSON, WJ
影响因子：
6.5
作者：
ELIAS, PM
通讯作者：
ELIAS, PM
影响因子：
4
作者：
Karin M. McCarthy;I. Skare;Michael C. Stankewich;M. Furuse;S. Tsukita;R. Rogers;R. Lynch;E. Schneeb
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Karin M. McCarthy;I. Skare;Michael C. Stankewich;M. Furuse;S. Tsukita;R. Rogers;R. Lynch;E. Schneeb
影响因子：
3.4
作者：
Stephen P. Sugrue;J. Zieske
通讯作者：
Stephen P. Sugrue;J. Zieske
影响因子：
56.9
作者：
Simon, DB;Lu, Y;Lifton, RP
通讯作者：
Lifton, RP