Systems identification and characterization of β-glucuronosyltransferase genes involved in arabinogalactan-protein biosynthesis in plant genomes.
Systems identification and characterization of β-glucuronosyltransferase genes involved in arabinogalactan-protein biosynthesis in plant genomes.
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DOI：
10.1038/s41598-020-72658-4
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发表时间：
2020-11-25
影响因子：
4.6
通讯作者：
Showalter AM
中科院分区：
文献类型：
--
作者：
Ajayi OO;Showalter AM
利用植物生物质生产生物乙醇需要了解植物细胞壁组装的分子机制。阿拉伯半乳聚糖蛋白（AGPs）是一类能与其他细胞壁聚合物相互作用，影响植物生长发育的糖蛋白。葡糖醛酸通过β-葡糖醛酸基转移酶（GLCAT）转移至AGP聚糖，是AGP中唯一能够结合钙的酸性糖。我们进行了全面的全基因组分析的一个假定的GLCAT基因家族参与AGP生物合成，通过检查其序列多样性，遗传结构，系统发育和模体特征，选择压力和基因表达在植物中。我们报告了161个分布在14个植物基因组和一个广泛保守的GLCAT催化结构域的GLCAT基因的鉴定。我们发现了一个系统发育的分支之间共享的brabetes和高等陆地植物的单子叶植物和双子叶植物谱系，并确定积极选择的网站，不导致功能分歧的GLCAT。对推定GLCAT基因的RNA-seq和微阵列数据分析揭示了可能影响植物细胞壁聚合物组装的基因表达特征，这对可食用和生物能源作物的整体生长和发育至关重要。
Utilizing plant biomass for bioethanol production requires an understanding of the molecular mechanisms involved in plant cell wall assembly. Arabinogalactan-proteins (AGPs) are glycoproteins that interact with other cell wall polymers to influence plant growth and developmental processes. Glucuronic acid, which is transferred to the AGP glycan by β-glucuronosyltransferases (GLCATs), is the only acidic sugar in AGPs with the ability to bind calcium. We carried out a comprehensive genome-wide analysis of a putative GLCAT gene family involved in AGP biosynthesis by examining its sequence diversity, genetic architecture, phylogenetic and motif characteristics, selection pressure and gene expression in plants. We report the identification of 161 putative GLCAT genes distributed across 14 plant genomes and a widely conserved GLCAT catalytic domain. We discovered a phylogenetic clade shared between bryophytes and higher land plants of monocot grass and dicot lineages and identified positively selected sites that do not result in functional divergence of GLCATs. RNA-seq and microarray data analyses of the putative GLCAT genes revealed gene expression signatures that likely influence the assembly of plant cell wall polymers which is critical to the overall growth and development of edible and bioenergy crops.
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影响因子：
5.6
作者：
Knoch E;Dilokpimol A;Geshi N
通讯作者：
Geshi N
两个层面的进化：基因和形态。
影响因子：
9.8
作者：
Carroll, SB
通讯作者：
Carroll, SB
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
DOI：
10.1073/pnas.0402177101
发表时间：
2004-08-31
影响因子：
11.1
作者：
Guindon, S;Rodrigo, AG;Huelsenbeck, JP
通讯作者：
Huelsenbeck, JP
影响因子：
64.5
作者：
CHEUNG, AY;WANG, H;WU, HM
通讯作者：
WU, HM