Structures of Class I and Class II Transcription Complexes Reveal the Molecular Basis of RamA-Dependent Transcription Activation.
Structures of Class I and Class II Transcription Complexes Reveal the Molecular Basis of RamA-Dependent Transcription Activation.
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DOI：
10.1002/advs.202103669
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Wang M
中科院分区：
文献类型：
--
作者：
Hao M;Ye F;Jovanovic M;Kotta-Loizou I;Xu Q;Qin X;Buck M;Zhang X;Wang M
转录激活因子拉马通过控制编码外排泵（acrA）和孔蛋白调节反义RNA（micF）的基因与肺炎克雷伯菌的多药耐药性相关。在细菌中，σ 70与激活剂一起通过将RNA聚合酶（RNAP）募集到启动子区域来控制大多数基因。RNAP和σ 70形成识别-35和-10启动子DNA共有位点的全酶。许多激活剂结合在全酶的上游，并且可以大致分为两类。拉马分别作为acrA上的I类激活剂和micF上的II类激活剂。作者提供了拉马在两个启动子处与RNAP-σ 70复合的生化和结构数据，这些数据揭示了拉马如何组装并与核心RNAP相互作用并激活转录从而导致抗生素耐药性的分子基础。此外，与CAP/TAP复合物的比较揭示了激活剂结合中的共同和激活剂特异性特征，并揭示了RNAP α亚基的两个C末端结构域的不同作用。转录激活因子拉马与肺炎克雷伯菌的多药耐药性有关这项研究揭示了拉马如何与RNA聚合酶（RNAP）相互作用并激活导致抗生素耐药性的转录的分子基础。此外，与分解代谢物激活蛋白/嗜热CAP同源物（CAP/TAP）复合物的比较揭示了激活剂结合中的共同和激活剂特异性特征，并揭示了RNAP的两个C末端结构域的不同作用。
Transcription activator RamA is linked to multidrug resistance of Klebsiella pneumoniae through controlling genes that encode efflux pumps (acrA) and porin‐regulating antisense RNA (micF). In bacteria, σ 70, together with activators, controls the majority of genes by recruiting RNA polymerase (RNAP) to the promoter regions. RNAP and σ 70 form a holoenzyme that recognizes ‐35 and ‐10 promoter DNA consensus sites. Many activators bind upstream from the holoenzyme and can be broadly divided into two classes. RamA acts as a class I activator on acrA and class II activator on micF, respectively. The authors present biochemical and structural data on RamA in complex with RNAP‐σ 70 at the two promoters and the data reveal the molecular basis for how RamA assembles and interacts with core RNAP and activates transcription that contributes to antibiotic resistance. Further, comparing with CAP/TAP complexes reveals common and activator‐specific features in activator binding and uncovers distinct roles of the two C‐terminal domains of RNAP α subunit. Transcription activator RamA is linked to multidrug resistance of Klebsiella pneumoniae. This study reveals the molecular basis for how RamA interacts with RNA polymerase (RNAP) and activates transcription that contributes to antibiotic resistance. Further, comparing with catabolite activator protein/thermophilic CAP homolog ( CAP/TAP) complexes reveals common and activator‐specific features in activator bindings and uncovers distinct roles of the two C‐terminal domains of RNAP.
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影响因子：
2.9
作者：
Schaefer J;Jovanovic G;Kotta-Loizou I;Buck M
通讯作者：
Buck M
DOI：
10.1073/pnas.95.18.10413
发表时间：
1998-09-01
影响因子：
11.1
作者：
Rhee, S;Martin, RG;Davies, DR
通讯作者：
Davies, DR
影响因子：
64.5
作者：
GASTON, K;BELL, A;BUSBY, S
通讯作者：
BUSBY, S
影响因子：
48
作者：
Punjani, Ali;Rubinstein, John L.;Brubaker, Marcus A.
通讯作者：
Brubaker, Marcus A.
DOI：
10.1126/science.aaf4417
发表时间：
2016-06-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Feng Y;Zhang Y;Ebright RH
通讯作者：
Ebright RH