Bioluminescent Multi-Characteristic Opsin for Simultaneous Optical Stimulation and Continuous Monitoring of Cortical Activities.
Bioluminescent Multi-Characteristic Opsin for Simultaneous Optical Stimulation and Continuous Monitoring of Cortical Activities.
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DOI：
10.3389/fncel.2021.750663
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发表时间：
2021
影响因子：
5.3
通讯作者：
Kumar Mohanty S
中科院分区：
文献类型：
--
作者：
Narcisse D;Mustafi SM;Carlson M;Batabyal S;Kim S;Wright W;Kumar Mohanty S
在细胞分辨率下刺激和连续监测神经活动是理解刺激的感觉处理和开发有效的神经调节疗法所必需的。我们提出了生物发光多特征视蛋白（bMCOII），一种混合光遗传学致动器，和生物发光Ca 2+传感器，用于无激发，连续监测视觉皮层中的神经活动，具有高时空分辨率。异常低强度（10 μW/mm 2）的光可引起神经激活，可通过Ca 2+生物发光成像检测到。对小鼠皮质中视觉诱发神经活动的不间断（>14小时）记录使得能够确定感觉激活的强度。此外，基于人工智能的神经激活参数将Ca 2+生物发光信号转换为网络活动模式。在连续的Ca 2 +-生物发光记录，视觉皮层活动在麻醉的第七至第八小时达到峰值，符合昼夜节律。对于皮层切片中的直接光遗传学刺激和视觉皮层中的视觉诱发活动，我们观察到二次延迟的Ca 2+生物发光反应，表明神经元-星形胶质细胞-神经元通路的参与。我们的方法将能够开发一个模块化和可扩展的接口系统，能够为多种应用程序提供服务，以调节和监测大脑中的大规模活动。
Stimulation and continuous monitoring of neural activities at cellular resolution are required for the understanding of the sensory processing of stimuli and development of effective neuromodulation therapies. We present bioluminescence multi-characteristic opsin (bMCOII), a hybrid optogenetic actuator, and a bioluminescence Ca2+ sensor for excitation-free, continuous monitoring of neural activities in the visual cortex, with high spatiotemporal resolution. An exceptionally low intensity (10 μW/mm2) of light could elicit neural activation that could be detected by Ca2+ bioluminescence imaging. An uninterrupted (>14 h) recording of visually evoked neural activities in the cortex of mice enabled the determination of strength of sensory activation. Furthermore, an artificial intelligence-based neural activation parameter transformed Ca2+ bioluminescence signals to network activity patterns. During continuous Ca2+-bioluminescence recordings, visual cortical activity peaked at the seventh to eighth hour of anesthesia, coinciding with circadian rhythm. For both direct optogenetic stimulation in cortical slices and visually evoked activities in the visual cortex, we observed secondary delayed Ca2+-bioluminescence responses, suggesting the involvement of neuron-astrocyte-neuron pathway. Our approach will enable the development of a modular and scalable interface system capable of serving a multiplicity of applications to modulate and monitor large-scale activities in the brain.
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影响因子：
--
作者：
Paladino N;Duhart JM;Mul Fedele ML;Golombek DA
通讯作者：
Golombek DA
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
7.7
作者：
Peterchev, Angel V.;Wagner, Timothy A.;Miranda, Pedro C.;Nitsche, Michael A.;Paulus, Walter;Lisanby, Sarah H.;Pascual-Leone, Alvaro;Bikson, Marom
通讯作者：
Bikson, Marom
影响因子：
5.3
作者：
Baker CK;Flannery JG
通讯作者：
Flannery JG
影响因子：
7.8
作者：
BENSON, DM;BRYAN, J;SMITH, LC
通讯作者：
SMITH, LC