North American House Sparrows Are Competent for Usutu Virus Transmission.
North American House Sparrows Are Competent for Usutu Virus Transmission.
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DOI：
10.1128/msphere.00295-22
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发表时间：
2022-12-21
期刊：
影响因子：
4.8
通讯作者：
Duggal, Nisha K.
中科院分区：
文献类型：
--
作者：
Kuchinsky, Sarah C.;Marano, Jeffrey;Hawks, Seth A.;Loessberg, Emma;Honaker, Christa F.;Siegel, Paul B.;Lahondere, Chloe;LeRoith, Tanya;Weger-Lucarelli, James;Duggal, Nisha K.
乌苏图病毒（USUV，黄病毒科）是一种新兴的蚊媒病毒，与人类神经侵袭性疾病和野鸟流行性死亡有关。 USUV 维持在嗜鸟蚊子（主要是库蚊属）和野鸟（主要是雀形目物种）之间的地方性动物循环中。然而，关于能够传输 USUV 的物种的实验数据有限。在这里，我们证明麻雀对多种 USUV 菌株敏感。我们的研究还表明，致倦库蚊对 USUV 敏感，荷兰 2016 年 USUV 毒株的感染率显着高于乌干达 2012 年 USUV 毒株，分别为 50% 和 19%。为了评估鸟类宿主和蚊媒之间的传播，我们让蚊子以接种了 USUV 的幼鸡或家麻雀为食。两种鸟类模型都将 USUV 传播给 C. quinquefasciatus 蚊子。线性回归分析表明，致倦线虫感染率与禽病毒血症水平呈正相关，3至4 log10 PFU/mL代表传播给蚊子的最低禽病毒血症阈值。根据传播所需的病毒血症，估计家麻雀比乌干达 2012 毒株更容易传播荷兰 2016 毒株。这些研究为 USUV 的有效储存宿主提供了见解。重要性 乌苏图病毒 (USUV) 是一种人畜共患的蚊媒病毒，可引起人类神经侵袭性疾病，包括脑膜炎和脑炎，并导致数十万野生鸟类死亡。 USUV 在自然界中的存在取决于库蚊属之间的传播。蚊子和各种鸟类。迄今为止，很少有实验数据可以确定哪些鸟类对于 USUV 的维护很重要。我们的研究表明，麻雀可以传播传染性乌苏图病毒，这表明它们作为宿主物种的作用。通过识别 USUV 的储存物种，我们可以预测 USUV 出现的区域并减轻其对全球人类和野生动物健康的影响。
Usutu virus (USUV, Flaviviridae) is an emerging mosquito-borne virus that has been implicated in neuroinvasive disease in humans and epizootic deaths in wild birds. USUV is maintained in an enzootic cycle between ornithophilic mosquitoes, primarily Culex spp., and wild birds, predominantly passerine species. However, limited experimental data exist on the species competent for USUV transmission. Here, we demonstrate that house sparrows are susceptible to multiple USUV strains. Our study also revealed that Culex quinquefasciatus mosquitoes are susceptible to USUV, with a significantly higher infection rate for the Netherlands 2016 USUV strain compared to the Uganda 2012 USUV strain at 50% and 19%, respectively. To assess transmission between avian host and mosquito vector, we allowed mosquitoes to feed on either juvenile chickens or house sparrows inoculated with USUV. Both bird models transmitted USUV to C. quinquefasciatus mosquitoes. Linear regression analyses indicated that C. quinquefasciatus infection rates were positively correlated with avian viremia levels, with 3 to 4 log10 PFU/mL representing the minimum avian viremia threshold for transmission to mosquitoes. Based on the viremia required for transmission, house sparrows were estimated to more readily transmit the Netherlands 2016 strain compared to the Uganda 2012 strain. These studies provide insights on a competent reservoir host of USUV. IMPORTANCE Usutu virus (USUV) is a zoonotic mosquito-borne virus that can cause neuroinvasive disease, including meningitis and encephalitis, in humans and has resulted in hundreds of thousands of deaths in wild birds. The perpetuation of USUV in nature is dependent on transmission between Culex spp. mosquitoes and various avian species. To date, few experimental data exist for determining which bird species are important for the maintenance of USUV. Our studies showed that house sparrows can transmit infectious Usutu virus, indicating their role as a competent host species. By identifying reservoir species of USUV, we can predict areas of USUV emergence and mitigate its impacts on global human and wildlife health.
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DOI：
10.2807/1560-7917.es.2020.25.41.2001732
发表时间：
2020-10
期刊：
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
影响因子：
--
作者：
Folly AJ;Lawson B;Lean FZ;McCracken F;Spiro S;John SK;Heaver JP;Seilern-Moy K;Masters N;Hernández-Triana LM;Phipps LP;Nuñez A;Fooks AR;Cunningham AA;Johnson N;McElhinney LM
通讯作者：
McElhinney LM
影响因子：
3.7
作者：
Calzolari M;Bonilauri P;Bellini R;Albieri A;Defilippo F;Maioli G;Galletti G;Gelati A;Barbieri I;Tamba M;Lelli D;Carra E;Cordioli P;Angelini P;Dottori M
通讯作者：
Dottori M
欧洲和非洲的新兴arbovirus忽视了USUTU病毒的进化史和扩散的重建。
影响因子：
6.4
作者：
Engel D;Jöst H;Wink M;Börstler J;Bosch S;Garigliany MM;Jöst A;Czajka C;Lühken R;Ziegler U;Groschup MH;Pfeffer M;Becker N;Cadar D;Schmidt-Chanasit J
通讯作者：
Schmidt-Chanasit J
影响因子：
11.8
作者：
Hayes EB;Komar N;Nasci RS;Montgomery SP;O'Leary DR;Campbell GL
通讯作者：
Campbell GL
影响因子：
3.3
作者：
Chvala, S.;Bakonyi, T.;Weissenboeck, H.
通讯作者：
Weissenboeck, H.