Evidence of simultaneous circulation of West Nile and Usutu viruses in mosquitoes sampled in Emilia-Romagna region (Italy) in 2009.
Evidence of simultaneous circulation of West Nile and Usutu viruses in mosquitoes sampled in Emilia-Romagna region (Italy) in 2009.
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DOI：
10.1371/journal.pone.0014324
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发表时间：
2010-12-15
期刊：
影响因子：
3.7
通讯作者：
Dottori M
中科院分区：
文献类型：
--
作者：
Calzolari M;Bonilauri P;Bellini R;Albieri A;Defilippo F;Maioli G;Galletti G;Gelati A;Barbieri I;Tamba M;Lelli D;Carra E;Cordioli P;Angelini P;Dottori M
近年来，在埃米利亚-罗马涅地区（意大利），由蚊媒病毒引起的人类疾病报告越来越多，从2007年的基孔肯雅病毒到2008年的西尼罗河病毒（WNV）。2009年进行了广泛的昆虫学调查，以确定该地区蚊媒病毒的存在和分布，特别是黄病毒。从5月6日到10月31日，在地理参考站共采集了190，516只蚊子，根据采集日期，地点和物种分为1，789个池，并通过逆转录聚合酶链反应（RT-PCR）进行分析，以检测属于黄病毒属的RNA的存在。在27个蚊池中检测到西尼罗河病毒，其序列与2008年爆发的鸟类和人类菌株相似，指出该病毒可能越冬。从其中一个池中分离出西尼罗河病毒。此外，有56个蚊子池的登革热病毒（USUV）检测呈阳性。大多数PCR阳性池由尖音库蚊组成，这也是分析最多的蚊子物种（81.4%的标本）;有趣的是，在两个白纹伊蚊蚊子池中也发现了USUVRNA。同时循环的西尼罗河病毒和USUV在调查区域突出了8蚊子西尼罗河病毒和USUV阳性池的发生和重叠的病毒“热点”，核密度估计（KDE）分析。土地利用的采样站指出，WNV阳性Cx的比例较高。农村环境中的pipiens池尊重总采样池的种源，而UUV阳性池在不同环境中均匀捕获。获得的数据突出了Cx的可能作用。在北方意大利，尖音蚊是传播西尼罗河病毒和USUV的主要媒介，Ae.白纹伊蚊在USUV循环中的作用。所描述的基于蚊子的监测可以构成针对蚊媒虫媒病毒的公共卫生警报系统的基础。
In recent years human diseases due to mosquito-borne viruses were increasingly reported in Emilia-Romagna region (Italy), from the chikungunya virus in 2007 to the West Nile virus (WNV) in 2008. An extensive entomological survey was performed in 2009 to establish the presence and distribution of mosquito arboviruses in this region, with particular reference to flaviviruses. From May 6 to October 31, a total of 190,516 mosquitoes were sampled in georeferenced stations, grouped in 1,789 pools according date of collection, location, and species, and analyzed by reverse transcription polymerase chain reaction (RT-PCR) to detect the presence of RNA belong to Flavivirus genus. WNV was detected in 27 mosquito pools, producing sequences similar to those of birds and human strains obtained in 2008 outbreak, pointed out the probable virus overwintering. Isolation of WNV was achieved from one of these pools. Moreover 56 pools of mosquitoes tested positive for Usutu virus (USUV). Most PCR positive pools consisted of Culex pipiens, which also was the most analyzed mosquito species (81.4% of specimens); interestingly, USUV RNA was also found in two Aedes albopictus mosquito pools. Simultaneous circulation of WNV and USUV in the survey area was highlighted by occurrence of 8 mosquito WNV- and USUV-positive pools and by the overlaying of the viruses “hot spots”, obtained by kernel density estimation (KDE) analysis. Land use of sampled stations pointed out a higher proportion of WNV-positive Cx. pipiens pool in rural environments respect the provenience of total sampled pool, while the USUV-positive pools were uniformly captured in the different environments. Obtained data highlighting the possible role of Cx. pipiens mosquito as the main vector for WNV and USUV in Northern Italy, and the possible involvement of Ae. albopictus mosquito in USUV cycle. The described mosquito-based surveillance could constitute the foundation for a public health alert system targeting mosquito borne arboviruses.
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影响因子：
4.8
作者：
Buckley A;Dawson A;Gould EA
通讯作者：
Gould EA
影响因子：
3.3
作者：
Chvala, S.;Bakonyi, T.;Weissenboeck, H.
通讯作者：
Weissenboeck, H.
影响因子：
3.7
作者：
Bakonyi, T;Gould, EA;Nowotny, N
通讯作者：
Nowotny, N
影响因子：
1
作者：
Chisenhall, Daniel M.;Vitek, Christopher J.;Mores, Christopher N.
通讯作者：
Mores, Christopher N.
DOI：
10.1016/j.trstmh.2008.03.019
发表时间：
2008-08-01
影响因子：
2.2
作者：
Gu, Weidong;Unnasch, Thomas R.;Novak, Robert J.
通讯作者：
Novak, Robert J.