Anion channels with multiple conductance levels in a mouse B lymphocyte cell line.
Anion channels with multiple conductance levels in a mouse B lymphocyte cell line.
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小鼠 B 淋巴细胞系中具有多种电导水平的阴离子通道。
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
M. Bosma
中科院分区：
文献类型：
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作者：
M. Bosma
1.从小鼠B淋巴细胞杂交瘤细胞中分离和贴壁细胞，记录到多个电导水平的离子通道。单通道电流的反转电位不受膜表面阳离子种类的影响，但随氯离子浓度的变化而变化，表明该通道具有阴离子选择性。2.由反转电位确定的渗透率序列为F-大于I-大于SCN-大于Br-大于Cl-大于葡萄糖醛酸大于NO3-大于天冬氨酸。这与电导顺序不同(Cl-大于SCN-=F-大于Br-大于NO3-大于I-大于葡萄糖醛酸大于天冬氨酸)，表明离子在通道的孔内相互作用。与此一致的是，用硫氰酸盐和氯化物的混合溶液观察到异常的摩尔分数依赖关系。3.除主要开放水平(约400ps；切除斑片，对称的165 mm-Cl-)外，还观察到三个亚导水平和一个超导水平。根据外观的重合性和相同的渗透率，这些都是同一通道的组成部分。4.通道是电压依赖的，正电位越大，切除斑块的开放概率越大。该通道被细胞质表面的二苯乙烯衍生物(SITS(4‐acetamido‐4‘‐isothiocyanostilbene‐2，2’‐disulphonic酸)以电压依赖的方式可逆地阻断。5.细胞贴片记录和摘除贴片记录之间存在一些差异。多电导水平通道在细胞贴附的贴片中较少出现，但经常可以通过长时间施加正电压而诱导出现。在贴片上的这种诱导通道表现出一种改变的电压依赖性，在切除的贴片中可以通过在细胞膜的细胞质一侧的溶液中加入环磷酸腺苷和三磷酸腺苷来部分模拟这种通道。
1. Multiple conductance level ion channels were recorded in excised and cell‐attached patches from cells of a mouse B lymphocyte hybridoma line. The reversal potential for the single‐channel current was unaffected by the species of cation on the cytoplasmic face of the patch, but changed as the Cl‐ concentration was altered, indicating that the channel is anion selective. 2. The permeability sequence determined from reversal potentials was F‐ greater than I‐ greater than SCN‐ greater than Br‐ greater than Cl‐ greater than glucuronate greater than NO3‐ greater than aspartate. This was different from the conductance sequence (Cl‐ greater than SCN‐ = F‐ greater than Br‐ greater than NO3‐ greater than I‐ greater than glucuronate greater than aspartate), indicating interaction of ions within the pore of the channel. Consistent with this was the observation of anomalous mole fraction dependence with a mixed solution of thiocyanate and chloride. 3. In addition to the main open level (about 400 pS; excised patch, symmetrical 165 mM‐Cl‐), three subconductance levels and one supraconductance level were observed. These were concluded to be integral components of the same channel based on coincidence of appearance and identical permeabilities. 4. The channel is voltage dependent, with open probability in excised patches increasing with more positive potentials. The channel was reversibly blocked in a voltage‐dependent manner by SITS (4‐acetamido‐4'‐isothiocyanostilbene‐2,2'‐disulphonic acid), a stilbene derivative, on the cytoplasmic face. 5. Several differences were noted between cell‐attached and excised‐patch recordings. The multiple conductance level channel was less frequently seen in cell‐attached patches but could often be induced to appear by prolonged application of positive voltages. This induced channel in attached patches showed an altered voltage dependence which could be partially mimicked in excised patches by including cyclic AMP and ATP in the solution on the cytoplasmic side of the membrane.
哺乳动物紧密上皮基底外侧膜中的单一阴离子选择性通道。
DOI：
10.1073/pnas.82.22.7791
发表时间：
1985
影响因子：
11.1
作者：
Hanrahan,JW;Alles,WP;Lewis,SA
通讯作者：
Lewis,SA
DOI：
10.1073/pnas.81.9.2772
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
MILLER, C;WHITE, MM
通讯作者：
WHITE, MM