Genetic Code Expansion: A Brief History and Perspective.
Genetic Code Expansion: A Brief History and Perspective.
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DOI：
10.1021/acs.biochem.1c00286
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发表时间：
2021-11-23
期刊：
影响因子：
2.9
通讯作者：
Cornish VW
中科院分区：
文献类型：
--
作者：
Shandell MA;Tan Z;Cornish VW
Since the establishment of site-specific mutagenesis of single amino acids to interrogate protein function in the 1970s, biochemists have sought to tailor protein structure in the native cell environment. Fine-tuning the chemical properties of proteins is an indispensable way to address fundamental mechanistic questions. Unnatural amino acids (UAAs) offer the possibility to expand beyond the 20 naturally occurring amino acids in most species and install new and useful chemical functions. Here, we review the literature about advances in UAA incorporation technology from chemoenzymatic aminoacylation of modified tRNAs to in vitro translation systems to genetic encoding of UAAs in the native cell environment and whole organisms. We discuss innovative applications of the UAA technology to challenges in bioengineering and medicine.
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DOI：
10.1073/pnas.48.6.1086
发表时间：
1962-01-01
影响因子：
11.1
作者：
CHAPEVILLE, F;EHRENSTEIN, GV;LIPMANN, F
通讯作者：
LIPMANN, F
DOI：
10.1073/pnas.1309584110
发表时间：
2013-07-16
影响因子：
11.1
作者：
Chatterjee, Abhishek;Xiao, Han;Schultz, Peter G.
通讯作者：
Schultz, Peter G.
影响因子：
16.6
作者：
Carlson, Erik D.;D'Aquino, Anne E.;Jewett, Michael C.
通讯作者：
Jewett, Michael C.
影响因子：
46.9
作者：
Barber KW;Muir P;Szeligowski RV;Rogulina S;Gerstein M;Sampson JR;Isaacs FJ;Rinehart J
通讯作者：
Rinehart J
影响因子：
15
作者：
Melo Czekster C;Robertson WE;Walker AS;Söll D;Schepartz A
通讯作者：
Schepartz A