Kinetics and thermodynamics of DNA hybridization on gold nanoparticles.
Kinetics and thermodynamics of DNA hybridization on gold nanoparticles.
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金纳米颗粒上 DNA 杂交的动力学和热力学
DOI：
10.1093/nar/gkp230
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发表时间：
2009-06
影响因子：
14.9
通讯作者：
Zhao XS
中科院分区：
文献类型：
--
作者：
Chen C;Wang W;Ge J;Zhao XS
研究了固定在金纳米颗粒（GNPs）表面的单链DNA与双链DNA的杂交及其随后解离成双链DNA的过程。基于杂交诱导的荧光变化，采用荧光检测测量熔解曲线、解离率和杂交率。为了考虑界面非均质性，对数据进行定量分析，提出了状态分布和速率分布两个分布函数。推导了DNA在GNPs上杂交和解离的反应激活焓和熵，并与溶液中相同量的DNA进行了比较。结果表明，GNPs与DNA之间的相互作用减少了能量屏障，加速了粘附DNA的解离。当ssDNA粘附在GNP表面的表面密度较低时，溶液中的ssDNA首先吸附在GNP表面，然后沿表面扩散直至与固定DNA杂交。我们还发现，DNA发夹的二级结构抑制了GNPs与DNA的相互作用，增强了与GNPs粘附的DNA发夹的稳定性。
Hybridization of single-stranded DNA immobilized on the surface of gold nanoparticles (GNPs) into double stranded DNA and its subsequent dissociation into ssDNA were investigated. Melting curves and rates of dissociation and hybridization were measured using fluorescence detection based on hybridization-induced fluorescence change. Two distribution functions, namely the state distribution and the rate distribution, were proposed in order to take interfacial heterogeneity into account and to quantitatively analyze the data. Reaction and activation enthalpies and entropies of DNA hybridization and dissociation on GNPs were derived and compared with the same quantities in solution. Our results show that the interaction between GNPs and DNA reduces the energetic barrier and accelerates the dissociation of adhered DNA. At low surface densities of ssDNA adhered to GNP surface, the primary reaction pathway is that ssDNA in solution first adsorbs onto the GNP, and then diffuses along the surface until hybridizing with an immobilized DNA. We also found that the secondary structure of a DNA hairpin inhibits the interaction between GNPs and DNA and enhances the stability of the DNA hairpin adhered to GNPs.
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影响因子：
3.4
作者：
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通讯作者：
McKenzie, SE
影响因子：
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作者：
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作者：
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