Rootstock-scion exchanging mRNAs participate in the pathways of amino acids and fatty acid metabolism in cucumber under early chilling stress.
Rootstock-scion exchanging mRNAs participate in the pathways of amino acids and fatty acid metabolism in cucumber under early chilling stress.
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DOI：
10.1093/hr/uhac031
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发表时间：
2022-02-19
影响因子：
8.7
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中科院分区：
文献类型：
--
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黄瓜（Cucumis sativus L.）经常经历寒冷胁迫，这限制了它的生长和生产力。嫁接被广泛用于利用强健的根系来提高非生物抗逆性，这表明远端器官之间存在系统的信号交流。据报道，当植物遭受低温胁迫时，mrna会进化出包括长距离信号传导在内的强化策略。然而，移动mrna在缓解嫁接黄瓜冷胁迫中的潜在功能尚不清楚。本文建立并综合分析了黄瓜和南瓜各嫁接组合在低温胁迫下的生理变化、移动mRNA谱、地上组织和地下组织的转录组学和代谢组学变化。低温诱导的南瓜移动mrna簇与差异表达基因和差异代谢产物之间的关系表明，4个关键的低温诱导南瓜移动mrna与异种嫁接黄瓜组织中甘氨酸、丝氨酸和苏氨酸的合成以及脂肪酸β-氧化降解代谢高度相关。通过对黄瓜幼苗中移动mrna、代谢物的潜在运输以及甘油磷脂代谢途径关键代谢物的外源应用的验证，证实了移动mrna在调节嫁接黄瓜的冷却反应中的作用。我们的研究结果建立了耐冷南瓜长距离mrna与耐冷黄瓜脂肪酸β-氧化降解代谢之间的联系。它们还有助于揭示接穗和砧木之间的信号相互作用机制，作为嫁接黄瓜实现耐冷性的手段。
Cucumber (Cucumis sativus L.) often experiences chilling stress that limits its growth and productivity. Grafting is widely used to improve abiotic stress resistance by exploiting a vigorous root system, suggesting there exists systemic signals communication between distant organs. mRNAs are reported to be evolving fortification strategies involving long-distance signaling when plants suffer from chilling stress. However, the potential function of mobile mRNAs in alleviating chilling stress in grafted cucumber is still unknown. Here, the physiological changes, mobile mRNA profiles, and transcriptomic and metabolomic changes in above- and underground tissues of all graft combinations of cucumber and pumpkin responding to chilling stress were established and analyzed comprehensively. The co-relationship between the cluster of chilling-induced pumpkin mobile mRNAs with differentially expressed genes and differentially intensive metabolites revealed that four key chilling-induced pumpkin mobile mRNAs were highly related to glycine, serine, and threonine synthesis and fatty acid β-oxidative degradation metabolism in cucumber tissues of heterografts. The verification of mobile mRNAs, potential transport of metabolites, and exogenous application of key metabolites of the glycerophospholipid metabolism pathway in cucumber seedlings confirmed the role of mobile mRNAs in regulating chilling responses in grafted cucumber. Our results build a link between the long-distance mRNAs of chilling-tolerant pumpkin and the fatty acid β-oxidative degradation metabolism of chilling-sensitive cucumber. They also help to uncover the mechanism of signaling interaction between scion and stock as a means of achieving chilling tolerance in grafted cucumber.
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影响因子：
3.3
作者：
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通讯作者：
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